PRIMER_OPT_SIZE=20
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
PRIMER_MAX_GC=80.0
PRIMER_MAX_SIZE=25
PRIMER_MAX_TM=63.0
PRIMER_MIN_SIZE=18
PRIMER_MIN_GC=20
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_SELF_ANY=12
PRIMER_SELF_END=6.99
MARKER_NAME=14.RHAP64FE2.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
INCLUDED_REGION=19,432
TARGET=44,46,AC
TARGET=92,33,CA
TARGET=140,26,AC
TARGET=170,14,CA
TARGET=211,20,AC
TARGET=238,26,AC
TARGET=336,14,CA
PRIMER_LEFT_EXPLAIN=considered 443, too many Ns 288, low tm 128, high tm 9, high end compl 12, ok 6
PRIMER_RIGHT_EXPLAIN=considered 599, too many Ns 324, low tm 273, ok 2
PRIMER_PAIR_EXPLAIN=considered 43, unacceptable product size 38, ok 5
PRIMER_PAIR_PENALTY=6.4586
PRIMER_LEFT_PENALTY=1.104149
PRIMER_RIGHT_PENALTY=5.354412
PRIMER_LEFT_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT=21,21
PRIMER_RIGHT=313,23
PRIMER_LEFT_TM=59.896
PRIMER_RIGHT_TM=57.646
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=6.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=8.4675
PRIMER_LEFT_1_PENALTY=3.113106
PRIMER_RIGHT_1_PENALTY=5.354412
PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_1=23,21
PRIMER_RIGHT_1=313,23
PRIMER_LEFT_1_TM=57.887
PRIMER_RIGHT_1_TM=57.646
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=5.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=8.7899
PRIMER_LEFT_2_PENALTY=1.104149
PRIMER_RIGHT_2_PENALTY=7.685778
PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
PRIMER_LEFT_2=21,21
PRIMER_RIGHT_2=288,25
PRIMER_LEFT_2_TM=59.896
PRIMER_RIGHT_2_TM=57.314
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=24.000
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=6.00
PRIMER_RIGHT_2_SELF_END=4.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=9.1042
PRIMER_LEFT_3_PENALTY=3.749784
PRIMER_RIGHT_3_PENALTY=5.354412
PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=313,23
PRIMER_LEFT_3_TM=61.750
PRIMER_RIGHT_3_TM=57.646
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=12.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=5.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=10.1984
PRIMER_LEFT_4_PENALTY=4.843946
PRIMER_RIGHT_4_PENALTY=5.354412
PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=313,23
PRIMER_LEFT_4_TM=62.844
PRIMER_RIGHT_4_TM=57.646
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=12.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
