PRIMER_EXPLAIN_FLAG=1
PRIMER_NUM_RETURN=1
PRIMER_OPT_SIZE=20
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_INTERNAL_OLIGO_SELF_END=8
PRIMER_INTERNAL_OLIGO_MAX_TM=100
PRIMER_INTERNAL_OLIGO_MIN_TM=0
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=25
PRIMER_OPT_TM=60.0
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_GC=0.0
PRIMER_MAX_GC=100.0
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_SALT_CONC=50.0
PRIMER_DNA_CONC=50.0
PRIMER_NUM_NS_ACCEPTED=0
PRIMER_SELF_ANY=12
PRIMER_SELF_END=8
PRIMER_PRODUCT_SIZE_RANGE=75-500
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
MARKER_NAME=test_01
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Should succeed
PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.346766
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT=18,20
PRIMER_RIGHT=123,20
PRIMER_INTERNAL_OLIGO=72,20
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.018
PRIMER_INTERNAL_OLIGO_TM=59.661
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
MARKER_NAME=test_02
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Should still succeed
PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.346766
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT=18,20
PRIMER_RIGHT=123,20
PRIMER_INTERNAL_OLIGO=72,20
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.018
PRIMER_INTERNAL_OLIGO_TM=59.661
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
MARKER_NAME=test_03
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
PRIMER_PICK_ANYWAY=1
PRIMER_COMMENT=Both primers should have high repeat similarity
PRIMER_WARNING=Left primer is unacceptable: High similarity to mispriming or mishyb library; Right primer is unacceptable: High similarity to mispriming or mishyb library
PRIMER_PAIR_EXPLAIN=considered 1, high mispriming library similarity 1, ok 0
=
MARKER_NAME=test_04
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
PRIMER_PICK_ANYWAY=1
PRIMER_COMMENT=Should succeed and both primers should have low repeat similarity
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.346766
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT=18,20
PRIMER_RIGHT=123,20
PRIMER_INTERNAL_OLIGO=72,20
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.018
PRIMER_INTERNAL_OLIGO_TM=59.661
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
MARKER_NAME=test_05
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_MISPRIMING_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Both primers should have high repeat similarity, but the explain line for right primers will not indicate this because mispriming is calculated "lazily" -- only if there are good left primers.
PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0
PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0
=
MARKER_NAME=test_06
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_LEFT_INPUT=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_INPUT=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_INPUT=GGAAGAGAGGGTCATGCAAC
PRIMER_PICK_ANYWAY=1
PRIMER_COMMENT=Primers should be ok, but not internal oligo
PRIMER_WARNING=Hybridization probe is unacceptable: High similarity to mispriming or mishyb library
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.346766
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT=18,20
PRIMER_RIGHT=123,20
PRIMER_INTERNAL_OLIGO=72,20
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.018
PRIMER_INTERNAL_OLIGO_TM=59.661
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
MARKER_NAME=test_07
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_PICK_ANYWAY=1
PRIMER_COMMENT=Primers should be ok, but not internal oligo
PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 63, ok 2334
PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, high repeat similarity 72, ok 2300
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360
PRIMER_PAIR_EXPLAIN=considered 5374476, unacceptable product size 2713327, high end compl 966, no internal oligo 2660183, ok 0
=
MARKER_NAME=test_08
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Ns.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTCTGATCATCTTCTGATCCAAGACCCGTGCTAGTCTACTGTATTCTACATGTACCCTTCTACAAGCTTTGAATTCCATAATCATCAGAGAATCCAGAAATTATTCATTATCACATAATAACAACACTCAAATATTTAAAGTTTTATGGTTGAATCTTTATGATTGAACCCATTCTATCAGGTGGTTATTTTATATCTAATAGAATTCATTTCAGGGTTACACAAAGGTCCGAATCTATCCATTTTACTGAG
PRIMER_COMMENT=Should fail because of high repeat similarity for all internal oligos
PRIMER_LEFT_EXPLAIN=considered 1712, high any compl 6, high end compl 24, high repeat similarity 26, ok 1656
PRIMER_RIGHT_EXPLAIN=considered 1712, high any compl 6, high end compl 25, high repeat similarity 28, ok 1653
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 2665, GC content failed 297, high any compl 15, high end compl 23, high repeat similarity 1970, ok 360
PRIMER_PAIR_EXPLAIN=considered 2737377, unacceptable product size 1340121, no internal oligo 1397256, ok 0
=
MARKER_NAME=test_09
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Should succeed
PRIMER_WARNING=Unrecognized character (X) in mispriming library not_for_production_Xs.lib
PRIMER_LEFT_EXPLAIN=considered 2424, ok 2424
PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
PRIMER_PAIR_EXPLAIN=considered 94, unacceptable product size 90, ok 4
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.346766
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_INTERNAL_OLIGO_PENALTY=0.339231
PRIMER_LEFT_SEQUENCE=CGACTCTAGAGGATCCCCCT
PRIMER_RIGHT_SEQUENCE=TAGCCTGCCTTTGCAAACTT
PRIMER_INTERNAL_OLIGO_SEQUENCE=GGAAGAGAGGGTCATGCAAC
PRIMER_LEFT=18,20
PRIMER_RIGHT=123,20
PRIMER_INTERNAL_OLIGO=72,20
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.018
PRIMER_INTERNAL_OLIGO_TM=59.661
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_INTERNAL_OLIGO_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
MARKER_NAME=test_10
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_MISPRIMING_LIBRARY=not_for_production_Xs.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Should fail (Xs are treated as Ns).
PRIMER_WARNING=Unrecognized character (X) in mispriming library not_for_production_Xs.lib
PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, high repeat similarity 2397, ok 0
PRIMER_RIGHT_EXPLAIN=considered 2424, ok 2424
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, ok 3463
PRIMER_PAIR_EXPLAIN=considered 32248, unacceptable product size 32248, ok 0
=
MARKER_NAME=test_11
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=1
PRIMER_MISPRIMING_LIBRARY=
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=not_for_production_Xs.lib
SEQUENCE=GCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCCTACATTTTAGCATCAGTGAGTACAGCATGCTTACTGGAAGAGAGGGTCATGCAACAGATTAGGAGGTAAGTTTGCAAAGGCAGGCTAAGGAGGAGACGCACTGAATGCCATGGTAAGAACTCTGGACATAAAAATATTGGAAGTTGTTGAGCAAGTAAAAAAATGTTTGGAAGTGTTACTTTAGCAATGGCAAGAATGATAGTATGGAATAGATTGGCAGAATGAAGGCAAAATGATTAGACATATTGCATTAAGGTAAAAAATGATAACTGAAGAATTATGTGCCACACTTATTAATAAGAAAGAATATGTGAACCTTGCAGATGTTTCCCTCTAGTAG
PRIMER_COMMENT=Should fail (Xs are treated as Ns).
PRIMER_WARNING=Unrecognized character (X) in internal oligo mishyb library not_for_production_Xs.lib (for internal oligo)
PRIMER_LEFT_EXPLAIN=considered 2424, high end compl 27, ok 2397
PRIMER_RIGHT_EXPLAIN=considered 2424, high end compl 52, ok 2372
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 3555, GC content failed 92, high end compl 75, high repeat similarity 3028, ok 360
PRIMER_PAIR_EXPLAIN=considered 5691246, unacceptable product size 2862933, high end compl 994, no internal oligo 2827319, ok 0
=
