PRIMER_SEQUENCE_ID=pair_1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib
PRIMER_INTERNAL_OLIGO_MISHYB_LIBRARY=primer_repeat_boundary2_lib
SEQUENCE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_MISPRIMING=1
PRIMER_INTERNAL_OLIGO_MAX_MISHYB=1
PRIMER_SELF_ANY=25
PRIMER_SELF_END=25
PRIMER_INTERNAL_OLIGO_SELF_ANY=25
PRIMER_INTERNAL_OLIGO_SELF_END=25
PRIMER_INTERNAL_OLIGO_MAX_TM=100
PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
PRIMER_INTERNAL_OLIGO_MIN_TM=0
PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_INTERNAL_OLIGO_MIN_GC=0
PRIMER_INTERNAL_OLIGO_MAX_GC=100
PRIMER_MAX_SIZE=20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MAX_TM=100
PRIMER_MIN_TM=0
PRIMER_OPT_TM=60
PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1
PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 163, high repeat similarity 121, ok 42
PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1
PRIMER_PAIR_PENALTY=63.5840
PRIMER_LEFT_PENALTY=31.846382
PRIMER_RIGHT_PENALTY=31.737633
PRIMER_INTERNAL_OLIGO_PENALTY=31.846382
PRIMER_LEFT_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
PRIMER_RIGHT_SEQUENCE=GCGCGCGCGCGCGCCGCGCG
PRIMER_INTERNAL_OLIGO_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
PRIMER_LEFT=0,20
PRIMER_RIGHT=181,20
PRIMER_INTERNAL_OLIGO=111,20
PRIMER_LEFT_TM=91.846
PRIMER_RIGHT_TM=91.738
PRIMER_INTERNAL_OLIGO_TM=91.846
PRIMER_LEFT_GC_PERCENT=100.000
PRIMER_RIGHT_GC_PERCENT=100.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=100.000
PRIMER_LEFT_SELF_ANY=20.00
PRIMER_RIGHT_SELF_ANY=14.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=20.00
PRIMER_LEFT_SELF_END=20.00
PRIMER_RIGHT_SELF_END=14.00
PRIMER_INTERNAL_OLIGO_SELF_END=20.00
PRIMER_LEFT_MISPRIMING_SCORE=0.00, test_1    * 2.0
PRIMER_RIGHT_MISPRIMING_SCORE=0.00, test_1    * 2.0
PRIMER_PAIR_MISPRIMING_SCORE=0.00, test_1    * 2.0
PRIMER_INTERNAL_OLIGO_MISHYB_SCORE=0.00, test_1    * 2.0
PRIMER_LEFT_END_STABILITY=13.4000
PRIMER_RIGHT_END_STABILITY=13.4000
PRIMER_PAIR_COMPL_ANY=17.00
PRIMER_PAIR_COMPL_END=17.00
PRIMER_PRODUCT_SIZE=182
=
PRIMER_SEQUENCE_ID=pair_0
PRIMER_COMMENT="Should fail because of high repeat similarity"
PRIMER_MAX_MISPRIMING=20
PRIMER_INTERNAL_OLIGO_MAX_MISHYB=20
SEQUENCE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0
PRIMER_RIGHT_EXPLAIN=considered 64, ok 64
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 144, ok 144
PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0
=
