PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=36-2000
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_GC_CLAMP=0
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=27
PRIMER_OPT_TM=60.0
PRIMER_MIN_TM=57.0
PRIMER_MAX_TM=63.0
PRIMER_MAX_DIFF_TM=100.0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_SALT_CONC=50.0
PRIMER_DNA_CONC=50.0
PRIMER_NUM_NS_ACCEPTED=0
PRIMER_SELF_ANY=8.00
PRIMER_SELF_END=3.00
PRIMER_MAX_POLY_X=5
PRIMER_LIBERAL_BASE=0
PRIMER_NUM_RETURN=1
PRIMER_FIRST_BASE_INDEX=0
PRIMER_MISPRIMING_LIBRARY=not_for_production.lib
PRIMER_MAX_MISPRIMING=11.00
PRIMER_OUTSIDE_PENALTY=20.0
PRIMER_INSIDE_PENALTY=1000.0
PRIMER_SEQUENCE_ID=AT3_X1
TARGET=298,45
SEQUENCE=TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTCCCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACTTTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCTCTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCAGGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCCATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTCCCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAACACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGACGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTACCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGTGCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
PRIMER_LEFT_EXPLAIN=considered 3504, overlap target 289, GC content failed 16, low tm 818, high tm 1648, ok 733
PRIMER_RIGHT_EXPLAIN=considered 3504, overlap target 289, low tm 655, high tm 1723, ok 837
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.1800
PRIMER_LEFT_PENALTY=1.599465
PRIMER_RIGHT_PENALTY=1.580558
PRIMER_LEFT_SEQUENCE=CCCTGTGGAAGATTAGCGG
PRIMER_RIGHT_SEQUENCE=GGGAAAGCTCACCCCTCTT
PRIMER_LEFT=279,19
PRIMER_RIGHT=361,19
PRIMER_LEFT_TM=60.599
PRIMER_RIGHT_TM=60.581
PRIMER_LEFT_GC_PERCENT=57.895
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=7.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=83
=
PRIMER_SEQUENCE_ID=AT3_X2
TARGET=298,371
SEQUENCE=AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCCAAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCCCTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTAGCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGTGGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAGGAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGGGAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCATTTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGAGGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTTCTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAGTATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACTGATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTTCCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTATGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTAAAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGCACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATCTATATGT
PRIMER_LEFT_EXPLAIN=considered 6612, overlap target 289, GC content failed 1, low tm 1035, high tm 3921, long poly-x seq 22, ok 1344
PRIMER_RIGHT_EXPLAIN=considered 6657, overlap target 289, GC content failed 114, low tm 1327, high tm 3439, high end compl 2, long poly-x seq 19, ok 1467
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=22.9341
PRIMER_LEFT_PENALTY=1.148919
PRIMER_RIGHT_PENALTY=21.785193
PRIMER_LEFT_SEQUENCE=TTGGCATTTTGTCTCCTTGC
PRIMER_RIGHT_SEQUENCE=GGCAGAAGACCTTTGGTCG
PRIMER_LEFT=278,20
PRIMER_RIGHT=688,19
PRIMER_LEFT_TM=61.149
PRIMER_RIGHT_TM=60.785
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=411
=
PRIMER_SEQUENCE_ID=AT3_X3
TARGET=298,220
SEQUENCE=CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAGAGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTTCTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTGTGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCACCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAGGTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAATCGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTGGTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGACCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTCACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATAGTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGCCCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACCAAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
PRIMER_LEFT_EXPLAIN=considered 5081, overlap target 289, GC content failed 13, low tm 1874, high tm 1352, long poly-x seq 25, ok 1528
PRIMER_RIGHT_EXPLAIN=considered 5254, overlap target 289, GC content failed 103, low tm 1706, high tm 1557, ok 1599
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=263.1257
PRIMER_LEFT_PENALTY=261.732281
PRIMER_RIGHT_PENALTY=1.393460
PRIMER_LEFT_SEQUENCE=CCAAACCCACCACCATTTTT
PRIMER_RIGHT_SEQUENCE=CAGGGGTAACATCTGCAACTC
PRIMER_LEFT=265,20
PRIMER_RIGHT=538,21
PRIMER_LEFT_TM=61.732
PRIMER_RIGHT_TM=59.607
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=260.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=274
=
PRIMER_SEQUENCE_ID=AT3_X3'
TARGET=298,142
SEQUENCE=AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTTCATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGGAAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGCTTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGGGAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAGGAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCCGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTTAACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTTGCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAATGGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTATTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGAATACAGGC
PRIMER_LEFT_EXPLAIN=considered 4474, overlap target 289, GC content failed 273, low tm 1464, high tm 1424, high repeat similarity 13, ok 1011
PRIMER_RIGHT_EXPLAIN=considered 4294, overlap target 289, GC content failed 209, low tm 512, high tm 2472, long poly-x seq 25, ok 787
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=128.4484
PRIMER_LEFT_PENALTY=127.241506
PRIMER_RIGHT_PENALTY=1.206859
PRIMER_LEFT_SEQUENCE=CACAGGTGAGTAGGTTTATTTTCTGTT
PRIMER_RIGHT_SEQUENCE=CTCCAGGGCCATTCTGAGT
PRIMER_LEFT=265,27
PRIMER_RIGHT=458,19
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=60.207
PRIMER_LEFT_GC_PERCENT=37.037
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=120.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=194
=
PRIMER_SEQUENCE_ID=AT3_X4
TARGET=298,395
SEQUENCE=GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCACAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATGAATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATATCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGTCTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCTGATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGCGTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTCCTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAGGTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGCTTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGATCTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTCTCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCAACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTTTTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCATGGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGTGGAGAGGAATTTGAAAGGGCATTGGAATTCA
PRIMER_LEFT_EXPLAIN=considered 6872, overlap target 289, GC content failed 226, low tm 1924, high tm 2932, long poly-x seq 17, ok 1484
PRIMER_RIGHT_EXPLAIN=considered 7004, overlap target 289, low tm 1493, high tm 3474, ok 1748
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=10.0214
PRIMER_LEFT_PENALTY=6.906112
PRIMER_RIGHT_PENALTY=3.115306
PRIMER_LEFT_SEQUENCE=TGTTCTTACTTTGTGATTCTCTTCC
PRIMER_RIGHT_SEQUENCE=GGAGGAAACTCCTTCCTAGACAA
PRIMER_LEFT=273,25
PRIMER_RIGHT=715,23
PRIMER_LEFT_TM=58.094
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=443
=
PRIMER_SEQUENCE_ID=AT3_X5
TARGET=298,69
SEQUENCE=TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCTGTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCCAAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTAGGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCATCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAGGTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTCTTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCAGGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTGCTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTACCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAGAAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGGGTCGC
PRIMER_LEFT_EXPLAIN=considered 3744, overlap target 289, GC content failed 148, low tm 1249, high tm 1208, ok 850
PRIMER_RIGHT_EXPLAIN=considered 3744, overlap target 289, low tm 874, high tm 1504, high end compl 8, ok 1069
PRIMER_PAIR_EXPLAIN=considered 21, high end compl 20, ok 1
PRIMER_PAIR_PENALTY=45.7837
PRIMER_LEFT_PENALTY=22.061137
PRIMER_RIGHT_PENALTY=23.722528
PRIMER_LEFT_SEQUENCE=AGACTTCTCCGGTCTTCCTTC
PRIMER_RIGHT_SEQUENCE=AAGAGAGTGGGGAAGGTGTACT
PRIMER_LEFT=276,21
PRIMER_RIGHT=389,22
PRIMER_LEFT_TM=58.939
PRIMER_RIGHT_TM=58.277
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=114
=
PRIMER_SEQUENCE_ID=AT3_X6
TARGET=298,181
SEQUENCE=CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTTACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCTTACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACCCAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGATTTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAGGTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCGCTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAAGTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAATGTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATACAAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGGGGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTAACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAAGGGAAGGGAGAG
PRIMER_LEFT_EXPLAIN=considered 4819, overlap target 244, low tm 1696, high tm 1512, ok 1367
PRIMER_RIGHT_EXPLAIN=considered 4414, overlap target 289, GC content failed 103, low tm 1578, high tm 1288, high repeat similarity 14, ok 1142
PRIMER_PAIR_EXPLAIN=considered 3, high end compl 2, ok 1
PRIMER_PAIR_PENALTY=65.9427
PRIMER_LEFT_PENALTY=1.577239
PRIMER_RIGHT_PENALTY=64.365428
PRIMER_LEFT_SEQUENCE=TTTATTCCCATGTGACCTGC
PRIMER_RIGHT_SEQUENCE=GGAAGAGGTGCAAAGAATAAGA
PRIMER_LEFT=278,20
PRIMER_RIGHT=503,22
PRIMER_LEFT_TM=58.423
PRIMER_RIGHT_TM=57.635
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=60.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=226
=
PRIMER_SEQUENCE_ID=CETP_X1
TARGET=298,122
SEQUENCE=AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGATGGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAATGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCTGGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACATACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACCATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAAGGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGTAAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAGGAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGAGTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTTCAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGATTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
PRIMER_LEFT_EXPLAIN=considered 4274, overlap target 289, GC content failed 68, low tm 569, high tm 2779, ok 569
PRIMER_RIGHT_EXPLAIN=considered 4274, overlap target 289, low tm 375, high tm 2978, ok 632
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=7.8629
PRIMER_LEFT_PENALTY=4.158608
PRIMER_RIGHT_PENALTY=3.704304
PRIMER_LEFT_SEQUENCE=CTTACACACCACTGCCTGATAA
PRIMER_RIGHT_SEQUENCE=GGCAGACAGATGCACTGATACTT
PRIMER_LEFT=276,22
PRIMER_RIGHT=442,23
PRIMER_LEFT_TM=57.841
PRIMER_RIGHT_TM=60.704
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
=
PRIMER_SEQUENCE_ID=CETP_X2
TARGET=298,119
SEQUENCE=CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCCATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGAGCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACCCCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTTGGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAGTGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAGTCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCCCCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGAACCAGAGCC
PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 123, GC content failed 7, low tm 374, high tm 2820, ok 754
PRIMER_RIGHT_EXPLAIN=considered 2584, overlap target 289, GC content failed 1, low tm 95, high tm 1901, ok 298
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=186.7490
PRIMER_LEFT_PENALTY=2.407131
PRIMER_RIGHT_PENALTY=184.341886
PRIMER_LEFT_SEQUENCE=ATCCACTGCCCTCCCTCT
PRIMER_RIGHT_SEQUENCE=AGCCAGGTCACACCCGAG
PRIMER_LEFT=280,18
PRIMER_RIGHT=443,18
PRIMER_LEFT_TM=59.593
PRIMER_RIGHT_TM=62.342
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=15.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=180.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
=
PRIMER_SEQUENCE_ID=CETP_X3
TARGET=298,139
SEQUENCE=TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGGATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGAGTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTAGGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGC
PRIMER_LEFT_EXPLAIN=considered 4444, overlap target 289, low tm 844, high tm 2220, ok 1091
PRIMER_RIGHT_EXPLAIN=considered 4444, overlap target 289, low tm 400, high tm 2950, ok 805
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=2.2342
PRIMER_LEFT_PENALTY=0.821928
PRIMER_RIGHT_PENALTY=1.412222
PRIMER_LEFT_SEQUENCE=CCTCACTTCCATTCCTTCCC
PRIMER_RIGHT_SEQUENCE=CATCAGCTGACAGGAATGCTT
PRIMER_LEFT=278,20
PRIMER_RIGHT=457,21
PRIMER_LEFT_TM=60.822
PRIMER_RIGHT_TM=60.412
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=180
=
PRIMER_SEQUENCE_ID=CETP_X4
TARGET=298,75
SEQUENCE=CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCAT
PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, low tm 382, high tm 2360, ok 773
PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 348, high tm 2565, ok 602
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.6924
PRIMER_LEFT_PENALTY=2.413192
PRIMER_RIGHT_PENALTY=1.279241
PRIMER_LEFT_SEQUENCE=GGGTCCTTGGCTCTTTCC
PRIMER_RIGHT_SEQUENCE=CCAGAGGACGCTTGACACAT
PRIMER_LEFT=280,18
PRIMER_RIGHT=392,20
PRIMER_LEFT_TM=59.587
PRIMER_RIGHT_TM=61.279
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=113
=
PRIMER_SEQUENCE_ID=CETP_X5
TARGET=298,92
SEQUENCE=GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCAGCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAGCCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGTCGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGCTTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCCATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATTAAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCCTTAAAACCAACCCTAGGATATAGATTCT
PRIMER_LEFT_EXPLAIN=considered 3974, overlap target 289, low tm 348, high tm 2707, ok 630
PRIMER_RIGHT_EXPLAIN=considered 3974, overlap target 289, GC content failed 34, low tm 889, high tm 1971, ok 791
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=6.9089
PRIMER_LEFT_PENALTY=4.729227
PRIMER_RIGHT_PENALTY=2.179675
PRIMER_LEFT_SEQUENCE=CATGTCGGGTCTCCCTGC
PRIMER_RIGHT_SEQUENCE=GGGCACAGGGTGGTGTACTT
PRIMER_LEFT=280,18
PRIMER_RIGHT=409,20
PRIMER_LEFT_TM=62.729
PRIMER_RIGHT_TM=62.180
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=130
=
PRIMER_SEQUENCE_ID=CETP_X6
TARGET=298,74
SEQUENCE=TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGTGTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGCCACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTCTTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACTAGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCAT
PRIMER_LEFT_EXPLAIN=considered 3794, overlap target 289, low tm 681, high tm 1966, ok 858
PRIMER_RIGHT_EXPLAIN=considered 3794, overlap target 289, low tm 274, high tm 2616, high any compl 2, high end compl 3, ok 610
PRIMER_PAIR_EXPLAIN=considered 40, high end compl 38, ok 2
PRIMER_PAIR_PENALTY=185.3939
PRIMER_LEFT_PENALTY=82.014189
PRIMER_RIGHT_PENALTY=103.379680
PRIMER_LEFT_SEQUENCE=CATTGTGGTGCTTGCTGC
PRIMER_RIGHT_SEQUENCE=ACAGGGAGTCAGCCAGCCT
PRIMER_LEFT=276,18
PRIMER_RIGHT=395,19
PRIMER_LEFT_TM=59.986
PRIMER_RIGHT_TM=62.380
PRIMER_LEFT_GC_PERCENT=55.556
PRIMER_RIGHT_GC_PERCENT=63.158
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=80.000000
PRIMER_RIGHT_POSITION_PENALTY=100.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=120
=
PRIMER_SEQUENCE_ID=CETP_X7
TARGET=298,65
SEQUENCE=TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCTGCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCTGGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTGCGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTAAGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTGC
PRIMER_LEFT_EXPLAIN=considered 3704, overlap target 289, low tm 262, high tm 2572, high end compl 3, ok 578
PRIMER_RIGHT_EXPLAIN=considered 3593, overlap target 289, GC content failed 15, low tm 752, high tm 1654, long poly-x seq 22, ok 861
PRIMER_PAIR_EXPLAIN=considered 102, high any compl 81, high end compl 20, ok 1
PRIMER_PAIR_PENALTY=141.5484
PRIMER_LEFT_PENALTY=140.588967
PRIMER_RIGHT_PENALTY=0.959447
PRIMER_LEFT_SEQUENCE=CTGGAAAGCACCTGCTCTGT
PRIMER_RIGHT_SEQUENCE=ATGCAGACAGAAACGCACTC
PRIMER_LEFT=271,20
PRIMER_RIGHT=382,20
PRIMER_LEFT_TM=60.589
PRIMER_RIGHT_TM=59.041
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=140.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=112
=
PRIMER_SEQUENCE_ID=CETP_X8
TARGET=298,96
SEQUENCE=GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCAGCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACAGACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTCCCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGACGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAGCCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGCCCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTCAACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCATTCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCACCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATTCGGCTTCTGTCATCCTCTACAGCAGTGGATTG
PRIMER_LEFT_EXPLAIN=considered 4014, overlap target 289, low tm 645, high tm 2223, ok 857
PRIMER_RIGHT_EXPLAIN=considered 3861, overlap target 289, GC content failed 8, low tm 269, high tm 2629, long poly-x seq 22, ok 644
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=3.5141
PRIMER_LEFT_PENALTY=0.186600
PRIMER_RIGHT_PENALTY=3.327467
PRIMER_LEFT_SEQUENCE=CGTTGATCTTTTCCTCCTGC
PRIMER_RIGHT_SEQUENCE=CCACCCTCCCACAACTCCT
PRIMER_LEFT=278,20
PRIMER_RIGHT=412,19
PRIMER_LEFT_TM=59.813
PRIMER_RIGHT_TM=62.327
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=63.158
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
=
PRIMER_SEQUENCE_ID=CETP_X9
TARGET=163,184
SEQUENCE=TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCCATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCCCAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTACAAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCCCGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTCCAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGCTGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAGGAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGTGAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGATGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTTTATTCAGCCGGGAGCATGGGTAAGACTC
PRIMER_LEFT_EXPLAIN=considered 3527, overlap target 272, low tm 323, high tm 2355, ok 577
PRIMER_RIGHT_EXPLAIN=considered 4589, overlap target 154, low tm 711, high tm 2838, ok 886
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=229.4979
PRIMER_LEFT_PENALTY=4.864632
PRIMER_RIGHT_PENALTY=224.633226
PRIMER_LEFT_SEQUENCE=AGCGTCCTGGCTTCCTCC
PRIMER_RIGHT_SEQUENCE=ATCCCCTAGCAGCCCAGC
PRIMER_LEFT=145,18
PRIMER_RIGHT=375,18
PRIMER_LEFT_TM=62.865
PRIMER_RIGHT_TM=62.633
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=220.000000
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
=
PRIMER_SEQUENCE_ID=CETP_X10
TARGET=235,55
SEQUENCE=CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCCGGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTTGGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTTTCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCAGTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCCTGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCACACAGCCAATAACACCACCAATGGCAACAATTATAACAG
PRIMER_LEFT_EXPLAIN=considered 2785, overlap target 100, low tm 259, high tm 1906, ok 520
PRIMER_RIGHT_EXPLAIN=considered 1498, overlap target 226, low tm 153, high tm 792, long poly-x seq 22, ok 305
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=227.4525
PRIMER_LEFT_PENALTY=4.533349
PRIMER_RIGHT_PENALTY=222.919106
PRIMER_LEFT_SEQUENCE=TCCAACTTCCTCATTTCTTTTC
PRIMER_RIGHT_SEQUENCE=ATAAACCCCACACAGGGGC
PRIMER_LEFT=213,22
PRIMER_RIGHT=319,19
PRIMER_LEFT_TM=57.467
PRIMER_RIGHT_TM=61.919
PRIMER_LEFT_GC_PERCENT=36.364
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=220.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=107
=
PRIMER_SEQUENCE_ID=CETP_X11
TARGET=125,169
SEQUENCE=CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCTCCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCTTCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCGGGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGGCTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
PRIMER_LEFT_EXPLAIN=considered 2831, overlap target 106, GC content failed 50, low tm 286, high tm 1982, ok 407
PRIMER_RIGHT_EXPLAIN=considered 2741, overlap target 116, GC content failed 42, low tm 336, high tm 1775, ok 472
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=125.4336
PRIMER_LEFT_PENALTY=2.311131
PRIMER_RIGHT_PENALTY=123.122481
PRIMER_LEFT_SEQUENCE=GGGACCCCTGTCTTCCAC
PRIMER_RIGHT_SEQUENCE=ACCACCTCTCCCTGCACC
PRIMER_LEFT=107,18
PRIMER_RIGHT=317,18
PRIMER_LEFT_TM=60.311
PRIMER_RIGHT_TM=61.122
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=120.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=211
=
PRIMER_SEQUENCE_ID=CETP_X12
TARGET=298,72
SEQUENCE=GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTCTCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAAAGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTCCCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGAGGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTGGATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGT
PRIMER_LEFT_EXPLAIN=considered 3686, overlap target 289, GC content failed 114, low tm 669, high tm 1809, long poly-x seq 14, ok 791
PRIMER_RIGHT_EXPLAIN=considered 3774, overlap target 289, low tm 619, high tm 2094, ok 772
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=4.6081
PRIMER_LEFT_PENALTY=1.522783
PRIMER_RIGHT_PENALTY=3.085335
PRIMER_LEFT_SEQUENCE=CAAATTTGGTTTCTCTCCCC
PRIMER_RIGHT_SEQUENCE=CCTTCTCTTCTCTGCAGCACAT
PRIMER_LEFT=278,20
PRIMER_RIGHT=391,22
PRIMER_LEFT_TM=58.477
PRIMER_RIGHT_TM=61.085
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
=
PRIMER_SEQUENCE_ID=CETP_X13
TARGET=298,38
SEQUENCE=CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAGGATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGGAAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAATCAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTTCTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
PRIMER_LEFT_EXPLAIN=considered 3282, overlap target 289, GC content failed 1, low tm 523, high tm 1815, long poly-x seq 22, ok 632
PRIMER_RIGHT_EXPLAIN=considered 3434, overlap target 289, low tm 586, high tm 1682, ok 877
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=126.6247
PRIMER_LEFT_PENALTY=123.526785
PRIMER_RIGHT_PENALTY=3.097964
PRIMER_LEFT_SEQUENCE=GGGTCAAATTATCATCGCTTTTT
PRIMER_RIGHT_SEQUENCE=CCCCAGTCTATCCAAGACTACCT
PRIMER_LEFT=269,23
PRIMER_RIGHT=358,23
PRIMER_LEFT_TM=60.527
PRIMER_RIGHT_TM=59.902
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=120.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=90
=
PRIMER_SEQUENCE_ID=CETP_X14
TARGET=298,77
SEQUENCE=GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCCCCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGGTTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCATCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAGAGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCTTCCCATTTCACCTTTT
PRIMER_LEFT_EXPLAIN=considered 3425, overlap target 52, GC content failed 3, low tm 235, high tm 2489, high end compl 2, long poly-x seq 23, ok 621
PRIMER_RIGHT_EXPLAIN=considered 1454, overlap target 289, low tm 83, high tm 882, ok 200
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=106.5730
PRIMER_LEFT_PENALTY=21.740693
PRIMER_RIGHT_PENALTY=84.832325
PRIMER_LEFT_SEQUENCE=CACCATGGGCATTTGATTG
PRIMER_RIGHT_SEQUENCE=GTTTCCCCTCCAGCCCAC
PRIMER_LEFT=278,19
PRIMER_RIGHT=396,18
PRIMER_LEFT_TM=60.741
PRIMER_RIGHT_TM=62.832
PRIMER_LEFT_GC_PERCENT=47.368
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20.000000
PRIMER_RIGHT_POSITION_PENALTY=80.000000
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
=
PRIMER_SEQUENCE_ID=CETP_X15
TARGET=254,90
SEQUENCE=GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCAAGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGCTAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCCACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
PRIMER_LEFT_EXPLAIN=considered 3343, overlap target 289, GC content failed 24, low tm 488, high tm 1840, long poly-x seq 24, ok 678
PRIMER_RIGHT_EXPLAIN=considered 3758, overlap target 245, GC content failed 1, low tm 356, high tm 2464, high repeat similarity 3, long poly-x seq 22, ok 667
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=427.7318
PRIMER_LEFT_PENALTY=104.061036
PRIMER_RIGHT_PENALTY=323.670770
PRIMER_LEFT_SEQUENCE=ACTGGGGCTCTGTCCCCT
PRIMER_RIGHT_SEQUENCE=AGGAACAGGGGCTGGTGA
PRIMER_LEFT=231,18
PRIMER_RIGHT=377,18
PRIMER_LEFT_TM=62.061
PRIMER_RIGHT_TM=61.671
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=100.000000
PRIMER_RIGHT_POSITION_PENALTY=320.000000
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=147
=
PRIMER_SEQUENCE_ID=CETP_X16
TARGET=298,79
SEQUENCE=GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGCACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAGTACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAGTGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAAGGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAACCCTCCAAGCTT
PRIMER_LEFT_EXPLAIN=considered 3689, overlap target 286, GC content failed 3, low tm 288, high tm 2492, long poly-x seq 22, ok 598
PRIMER_RIGHT_EXPLAIN=considered 3637, overlap target 289, GC content failed 3, low tm 299, high tm 2316, long poly-x seq 25, ok 705
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=4.9909
PRIMER_LEFT_PENALTY=2.381034
PRIMER_RIGHT_PENALTY=2.609852
PRIMER_LEFT_SEQUENCE=CTCCTACTGCCCCTCCCTTC
PRIMER_RIGHT_SEQUENCE=CCCGACCTCCTTGGAGAC
PRIMER_LEFT=278,20
PRIMER_RIGHT=394,18
PRIMER_LEFT_TM=62.381
PRIMER_RIGHT_TM=60.610
PRIMER_LEFT_GC_PERCENT=65.000
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=117
=
PRIMER_SEQUENCE_ID=F2_X1
TARGET=298,114
SEQUENCE=AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCACTGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTTTTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATGGTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCTCACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGACAATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCCTGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGTGCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTGGCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
PRIMER_LEFT_EXPLAIN=considered 4041, overlap target 289, GC content failed 21, low tm 498, high tm 2476, long poly-x seq 22, ok 735
PRIMER_RIGHT_EXPLAIN=considered 4194, overlap target 289, GC content failed 8, low tm 235, high tm 3082, high repeat similarity 2, ok 578
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=25.9872
PRIMER_LEFT_PENALTY=2.861995
PRIMER_RIGHT_PENALTY=23.125223
PRIMER_LEFT_SEQUENCE=CCAGAGGGGTCAGGACAG
PRIMER_RIGHT_SEQUENCE=AGCCTGCAAGCACTCCCT
PRIMER_LEFT=280,18
PRIMER_RIGHT=430,18
PRIMER_LEFT_TM=59.138
PRIMER_RIGHT_TM=61.125
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=151
=
PRIMER_SEQUENCE_ID=F2_X2
TARGET=298,165
SEQUENCE=AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAGATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAGTGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCTTCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCTACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGGACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAGGAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAGGGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCCTGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAGCTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
PRIMER_LEFT_EXPLAIN=considered 4542, overlap target 289, GC content failed 14, low tm 245, high tm 3332, long poly-x seq 23, ok 639
PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 96, low tm 191, high tm 3583, high end compl 4, ok 541
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=1004.4866
PRIMER_LEFT_PENALTY=302.054062
PRIMER_RIGHT_PENALTY=702.432556
PRIMER_LEFT_SEQUENCE=GTCGCTGTTCCATGACCC
PRIMER_RIGHT_SEQUENCE=GTCTAGAGTTGGGCCCGGT
PRIMER_LEFT=265,18
PRIMER_RIGHT=516,19
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=61.433
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=63.158
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=7.00, reverse ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_MISPRIMING_SCORE=15.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=300.000000
PRIMER_RIGHT_POSITION_PENALTY=700.000000
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
=
PRIMER_SEQUENCE_ID=F2_X3
TARGET=298,29
SEQUENCE=TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGCCCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTCCCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACCTCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACATTTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCA
PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 7, low tm 659, high tm 1679, ok 710
PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, GC content failed 1, low tm 379, high tm 2040, ok 635
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=2.4864
PRIMER_LEFT_PENALTY=0.475706
PRIMER_RIGHT_PENALTY=2.010701
PRIMER_LEFT_SEQUENCE=ACTCCCAGCCCTTGTTTTTC
PRIMER_RIGHT_SEQUENCE=AAATCCTTCCCGGTGCTC
PRIMER_LEFT=278,20
PRIMER_RIGHT=344,18
PRIMER_LEFT_TM=60.476
PRIMER_RIGHT_TM=60.011
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=55.556
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=67
=
PRIMER_SEQUENCE_ID=F2_X4
TARGET=298,55
SEQUENCE=GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAACTGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGGTTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCCTGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAATGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
PRIMER_LEFT_EXPLAIN=considered 3604, overlap target 289, GC content failed 1, low tm 409, high tm 2209, high any compl 3, high end compl 14, ok 679
PRIMER_RIGHT_EXPLAIN=considered 3497, overlap target 289, GC content failed 107, low tm 371, high tm 2132, long poly-x seq 18, ok 580
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=123.3195
PRIMER_LEFT_PENALTY=121.626330
PRIMER_RIGHT_PENALTY=1.693157
PRIMER_LEFT_SEQUENCE=CCAAAGGTAAACACCTGGGTC
PRIMER_RIGHT_SEQUENCE=CAAACCCGTGTCAGTTGCTC
PRIMER_LEFT=271,21
PRIMER_RIGHT=372,20
PRIMER_LEFT_TM=60.626
PRIMER_RIGHT_TM=61.693
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=120.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
=
PRIMER_SEQUENCE_ID=F2_X5
TARGET=298,110
SEQUENCE=TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTATTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATATTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGGTGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
PRIMER_LEFT_EXPLAIN=considered 4154, overlap target 289, GC content failed 2, low tm 468, high tm 2494, ok 901
PRIMER_RIGHT_EXPLAIN=considered 4154, overlap target 289, GC content failed 14, low tm 310, high tm 2916, ok 625
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=328.5545
PRIMER_LEFT_PENALTY=323.965248
PRIMER_RIGHT_PENALTY=4.589237
PRIMER_LEFT_SEQUENCE=CAGGCTCCAAGGCTGACC
PRIMER_RIGHT_SEQUENCE=AAGGCCGACCCCTCACTC
PRIMER_LEFT=264,18
PRIMER_RIGHT=425,18
PRIMER_LEFT_TM=61.965
PRIMER_RIGHT_TM=62.589
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=320.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=162
=
PRIMER_SEQUENCE_ID=F2_X6
TARGET=298,141
SEQUENCE=GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTGGCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAGGGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAATTGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAATCGCTTGAACCCGGGAG
PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 2, low tm 392, high tm 2999, ok 782
PRIMER_RIGHT_EXPLAIN=considered 4464, overlap target 289, GC content failed 40, low tm 325, high tm 3245, ok 565
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=1184.0873
PRIMER_LEFT_PENALTY=0.806671
PRIMER_RIGHT_PENALTY=1183.280619
PRIMER_LEFT_SEQUENCE=GTTGGGCAATTTCCTCTTCC
PRIMER_RIGHT_SEQUENCE=CTTGGTTCCCATCCCAGG
PRIMER_LEFT=278,20
PRIMER_RIGHT=515,18
PRIMER_LEFT_TM=60.807
PRIMER_RIGHT_TM=61.281
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=1180.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=238
=
PRIMER_SEQUENCE_ID=F2_X7
TARGET=298,319
SEQUENCE=GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTACTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAACAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGAGGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGTCTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAGGCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGTCACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGGTGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCAAGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACGGGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCGACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCC
PRIMER_LEFT_EXPLAIN=considered 6244, overlap target 289, GC content failed 60, low tm 973, high tm 3795, ok 1127
PRIMER_RIGHT_EXPLAIN=considered 5938, overlap target 289, GC content failed 51, low tm 580, high tm 3983, long poly-x seq 44, ok 991
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=369.2002
PRIMER_LEFT_PENALTY=224.254433
PRIMER_RIGHT_PENALTY=144.945754
PRIMER_LEFT_SEQUENCE=CTCTGCTGCCTCCTTGCC
PRIMER_RIGHT_SEQUENCE=CTCAGGCCCCCTACCCAG
PRIMER_LEFT=269,18
PRIMER_RIGHT=641,18
PRIMER_LEFT_TM=62.254
PRIMER_RIGHT_TM=62.946
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=72.222
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=220.000000
PRIMER_RIGHT_POSITION_PENALTY=140.000000
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=373
=
PRIMER_SEQUENCE_ID=F2_X8
TARGET=298,133
SEQUENCE=GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGG
PRIMER_LEFT_EXPLAIN=considered 4078, overlap target 289, GC content failed 87, low tm 484, high tm 2399, high end compl 1, long poly-x seq 44, ok 774
PRIMER_RIGHT_EXPLAIN=considered 4384, overlap target 289, GC content failed 97, low tm 244, high tm 3201, ok 553
PRIMER_PAIR_EXPLAIN=considered 14, high end compl 12, ok 2
PRIMER_PAIR_PENALTY=706.6583
PRIMER_LEFT_PENALTY=683.278099
PRIMER_RIGHT_PENALTY=23.380210
PRIMER_LEFT_SEQUENCE=GGATGGGTGAGGAATGGC
PRIMER_RIGHT_SEQUENCE=CCTCGGATGCCCACTACCT
PRIMER_LEFT=246,18
PRIMER_RIGHT=450,19
PRIMER_LEFT_TM=61.278
PRIMER_RIGHT_TM=62.380
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=63.158
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=680.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=205
=
PRIMER_SEQUENCE_ID=F2_X9
TARGET=298,131
SEQUENCE=CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGCATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTTTTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATCTCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTCTCTTGCC
PRIMER_LEFT_EXPLAIN=considered 4212, overlap target 289, GC content failed 182, low tm 233, high tm 2939, long poly-x seq 22, ok 547
PRIMER_RIGHT_EXPLAIN=considered 4248, overlap target 289, GC content failed 80, low tm 415, high tm 2693, long poly-x seq 23, ok 748
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=166.1676
PRIMER_LEFT_PENALTY=2.845950
PRIMER_RIGHT_PENALTY=163.321659
PRIMER_LEFT_SEQUENCE=ACTGCTTGGCTTGCTCTGC
PRIMER_RIGHT_SEQUENCE=AATGGTAGCGCAGGGCTC
PRIMER_LEFT=279,19
PRIMER_RIGHT=454,18
PRIMER_LEFT_TM=61.846
PRIMER_RIGHT_TM=61.322
PRIMER_LEFT_GC_PERCENT=57.895
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=160.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
=
PRIMER_SEQUENCE_ID=F2_X10
TARGET=298,172
SEQUENCE=CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTGGGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGGCAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATCTGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGGGCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAGGCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAGTGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTTCACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGGTGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGTGGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTCTGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCTGTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
PRIMER_LEFT_EXPLAIN=considered 4774, overlap target 289, GC content failed 210, low tm 485, high tm 3153, ok 637
PRIMER_RIGHT_EXPLAIN=considered 4621, overlap target 289, GC content failed 8, low tm 474, high tm 3110, long poly-x seq 22, ok 718
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=105.4722
PRIMER_LEFT_PENALTY=101.889081
PRIMER_RIGHT_PENALTY=3.583075
PRIMER_LEFT_SEQUENCE=CTAATGCTTCCTGCTGCCC
PRIMER_RIGHT_SEQUENCE=ACGGGCCACCAGTTCTGT
PRIMER_LEFT=274,19
PRIMER_RIGHT=487,18
PRIMER_LEFT_TM=60.889
PRIMER_RIGHT_TM=61.583
PRIMER_LEFT_GC_PERCENT=57.895
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=100.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=214
=
PRIMER_SEQUENCE_ID=F2_X11
TARGET=298,178
SEQUENCE=TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCCCTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCCTATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGTTTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAGGTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTACAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGCCTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGCCACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAAGTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAAAGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGTTCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
PRIMER_LEFT_EXPLAIN=considered 4834, overlap target 289, low tm 620, high tm 3010, ok 915
PRIMER_RIGHT_EXPLAIN=considered 4762, overlap target 289, GC content failed 176, low tm 739, high tm 2528, long poly-x seq 13, ok 1017
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=4.7032
PRIMER_LEFT_PENALTY=0.594713
PRIMER_RIGHT_PENALTY=4.108476
PRIMER_LEFT_SEQUENCE=TTCTTTCTTGGGGTCTCTGC
PRIMER_RIGHT_SEQUENCE=ACAAGCATCTGGTGGCCC
PRIMER_LEFT=278,20
PRIMER_RIGHT=493,18
PRIMER_LEFT_TM=59.405
PRIMER_RIGHT_TM=62.108
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=216
=
PRIMER_SEQUENCE_ID=F2_X12
TARGET=298,186
SEQUENCE=CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTCGAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGCGTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGTTGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAGCTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTGGACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGTGGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGCTGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGTTGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGTTTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAATGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCAGCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAGCG
PRIMER_LEFT_EXPLAIN=considered 4914, overlap target 289, GC content failed 56, low tm 524, high tm 3341, ok 704
PRIMER_RIGHT_EXPLAIN=considered 4914, overlap target 289, GC content failed 83, low tm 976, high tm 2660, ok 906
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=286.8952
PRIMER_LEFT_PENALTY=2.027407
PRIMER_RIGHT_PENALTY=284.867800
PRIMER_LEFT_SEQUENCE=CCCTTCTTCCTTCCCCAA
PRIMER_RIGHT_SEQUENCE=CACTGTTCCCTCAGCCCG
PRIMER_LEFT=280,18
PRIMER_RIGHT=515,18
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=62.868
PRIMER_LEFT_GC_PERCENT=55.556
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=280.000000
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=236
=
PRIMER_SEQUENCE_ID=F2_X13
TARGET=298,75
SEQUENCE=AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGAATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTTGGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGGAAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTC
PRIMER_LEFT_EXPLAIN=considered 3804, overlap target 289, GC content failed 12, low tm 652, high tm 1841, ok 1010
PRIMER_RIGHT_EXPLAIN=considered 3804, overlap target 289, low tm 873, high tm 1715, high end compl 5, ok 922
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=62.8220
PRIMER_LEFT_PENALTY=0.838952
PRIMER_RIGHT_PENALTY=61.983042
PRIMER_LEFT_SEQUENCE=CAGACGGCTGTTCTCTTTCA
PRIMER_RIGHT_SEQUENCE=GCCCTGGGCTTTAGAGAAG
PRIMER_LEFT=278,20
PRIMER_RIGHT=394,19
PRIMER_LEFT_TM=59.161
PRIMER_RIGHT_TM=59.017
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=60.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=117
=
PRIMER_SEQUENCE_ID=F2_X14
TARGET=298,148
SEQUENCE=ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCCCGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGCGAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTAATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGCACTCGGGAGGCTGAGGTGGGAGGAT
PRIMER_LEFT_EXPLAIN=considered 4475, overlap target 230, low tm 988, high tm 2162, ok 1095
PRIMER_RIGHT_EXPLAIN=considered 3944, overlap target 289, GC content failed 74, low tm 898, high tm 1848, high end compl 2, ok 833
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=24.4497
PRIMER_LEFT_PENALTY=4.108177
PRIMER_RIGHT_PENALTY=20.341522
PRIMER_LEFT_SEQUENCE=TTGGAAACCTCATCTTTCTTCTTC
PRIMER_RIGHT_SEQUENCE=GAGCCCAGAATATGAGTGGC
PRIMER_LEFT=274,24
PRIMER_RIGHT=466,20
PRIMER_LEFT_TM=60.108
PRIMER_RIGHT_TM=59.658
PRIMER_LEFT_GC_PERCENT=37.500
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=193
=
PRIMER_SEQUENCE_ID=F2R_X1
TARGET=235,90
SEQUENCE=CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCCCCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGGTGAGA
PRIMER_LEFT_EXPLAIN=considered 2940, GC content failed 829, low tm 31, high tm 1984, ok 96
PRIMER_RIGHT_EXPLAIN=considered 951, overlap target 226, GC content failed 209, high tm 510, ok 6
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=57988.5264
PRIMER_LEFT_PENALTY=984.110493
PRIMER_RIGHT_PENALTY=57004.415904
PRIMER_LEFT_SEQUENCE=GAGGGTGAAGCGGAGCAG
PRIMER_RIGHT_SEQUENCE=GCCGCACAGACTGAAGCA
PRIMER_LEFT=168,18
PRIMER_RIGHT=285,18
PRIMER_LEFT_TM=62.110
PRIMER_RIGHT_TM=62.416
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=980.000000
PRIMER_RIGHT_POSITION_PENALTY=57000.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=118
=
PRIMER_SEQUENCE_ID=F2R_X2-seg1
TARGET=4,702
SEQUENCE=TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
PRIMER_LEFT_EXPLAIN=considered 6710, GC content failed 4, low tm 1539, high tm 3376, high repeat similarity 1, ok 1790
PRIMER_RIGHT_EXPLAIN=considered 6710, GC content failed 4, low tm 1540, high tm 3376, high repeat similarity 2, ok 1788
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=39005.2676
PRIMER_LEFT_PENALTY=19005.004751
PRIMER_RIGHT_PENALTY=20000.262854
PRIMER_LEFT_SEQUENCE=TTTCAGAATCAAAAGCAACAAAT
PRIMER_RIGHT_SEQUENCE=AGCAGGGTTTCATTGAGCAC
PRIMER_LEFT=0,23
PRIMER_RIGHT=705,20
PRIMER_LEFT_TM=57.995
PRIMER_RIGHT_TM=60.263
PRIMER_LEFT_GC_PERCENT=26.087
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=19000.000000
PRIMER_RIGHT_POSITION_PENALTY=20000.000000
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=706
=
PRIMER_SEQUENCE_ID=F2R_X2-seg2
TARGET=0,514
SEQUENCE=TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAAAGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATTGATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTCAAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCAAGGGAATATTGCCAATGCTACAGTAATAAA
PRIMER_LEFT_EXPLAIN=considered 4999, overlap target 227, low tm 1458, high tm 1982, long poly-x seq 22, ok 1310
PRIMER_RIGHT_EXPLAIN=considered 6997, GC content failed 109, low tm 2483, high tm 2360, long poly-x seq 22, ok 2023
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=20001.7104
PRIMER_LEFT_PENALTY=20000.498267
PRIMER_RIGHT_PENALTY=1.212099
PRIMER_LEFT_SEQUENCE=TGATGTGCTCAATGAAACCC
PRIMER_RIGHT_SEQUENCE=CTCCCAGCAGTCCCTTTTC
PRIMER_LEFT=0,20
PRIMER_RIGHT=532,19
PRIMER_LEFT_TM=59.502
PRIMER_RIGHT_TM=59.788
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=533
=
PRIMER_SEQUENCE_ID=F3_X1
TARGET=298,104
SEQUENCE=ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAGTGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGCAGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAGACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCCAACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGACATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTCCTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTGGAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCGCCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCACCCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGTTGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAATTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
PRIMER_LEFT_EXPLAIN=considered 4094, overlap target 289, GC content failed 1473, low tm 23, high tm 2263, ok 46
PRIMER_RIGHT_EXPLAIN=considered 4094, overlap target 289, GC content failed 716, low tm 101, high tm 2783, ok 205
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=808.3290
PRIMER_LEFT_PENALTY=4.915955
PRIMER_RIGHT_PENALTY=803.413051
PRIMER_LEFT_SEQUENCE=CTCGCCGCCAACTGGTAG
PRIMER_RIGHT_SEQUENCE=ACTGTCGCCTCCCTCCTG
PRIMER_LEFT=280,18
PRIMER_RIGHT=459,18
PRIMER_LEFT_TM=62.916
PRIMER_RIGHT_TM=61.413
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=16.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=800.000000
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=180
=
PRIMER_SEQUENCE_ID=F3_X2
TARGET=298,116
SEQUENCE=CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAAAATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATTCTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTTGTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACATCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAGGCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAATTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTGGGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAATGTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCTTGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAACTGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGGAGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
PRIMER_LEFT_EXPLAIN=considered 4082, overlap target 289, GC content failed 83, low tm 1878, high tm 779, high repeat similarity 1, long poly-x seq 20, ok 1032
PRIMER_RIGHT_EXPLAIN=considered 4214, overlap target 289, GC content failed 232, low tm 1968, high tm 682, ok 1043
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=113.6635
PRIMER_LEFT_PENALTY=109.223369
PRIMER_RIGHT_PENALTY=4.440107
PRIMER_LEFT_SEQUENCE=CATGTAATAATAGCTGTTTCTCTGTTG
PRIMER_RIGHT_SEQUENCE=TTTTCTTTTTCTGTACCCAGCTT
PRIMER_LEFT=266,27
PRIMER_RIGHT=436,23
PRIMER_LEFT_TM=57.777
PRIMER_RIGHT_TM=58.560
PRIMER_LEFT_GC_PERCENT=33.333
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=100.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=171
=
PRIMER_SEQUENCE_ID=F3_X3
TARGET=298,204
SEQUENCE=TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTGTACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACCCCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCATTAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTATCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAGCACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCTCACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCCGGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGAGTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGAAACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTATTCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTAGGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCCCCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAGAGGGCGGGCCAGGGAAGCGG
PRIMER_LEFT_EXPLAIN=considered 4965, overlap target 289, GC content failed 54, low tm 1212, high tm 2106, high repeat similarity 28, long poly-x seq 22, ok 1254
PRIMER_RIGHT_EXPLAIN=considered 5005, overlap target 289, GC content failed 185, low tm 1231, high tm 1945, long poly-x seq 11, ok 1344
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=209.0773
PRIMER_LEFT_PENALTY=206.472471
PRIMER_RIGHT_PENALTY=2.604792
PRIMER_LEFT_SEQUENCE=TTCCATTTATGTTTCTTCCTTCTT
PRIMER_RIGHT_SEQUENCE=TATTACAGCCCAAGCCACTT
PRIMER_LEFT=264,24
PRIMER_RIGHT=521,20
PRIMER_LEFT_TM=57.528
PRIMER_RIGHT_TM=57.395
PRIMER_LEFT_GC_PERCENT=29.167
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=200.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
=
PRIMER_SEQUENCE_ID=F3_X4
TARGET=298,183
SEQUENCE=TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAATGTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGCACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAGCTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAACAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAGCAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGACCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATGTTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGGTGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAACCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAGAAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCTGCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGCTGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
PRIMER_LEFT_EXPLAIN=considered 4884, overlap target 289, GC content failed 320, low tm 1911, high tm 1322, high end compl 6, ok 1036
PRIMER_RIGHT_EXPLAIN=considered 4770, overlap target 289, GC content failed 176, low tm 1330, high tm 1913, long poly-x seq 21, ok 1041
PRIMER_PAIR_EXPLAIN=considered 36, high end compl 33, ok 3
PRIMER_PAIR_PENALTY=330.1585
PRIMER_LEFT_PENALTY=2.108929
PRIMER_RIGHT_PENALTY=328.049552
PRIMER_LEFT_SEQUENCE=CCAAGCTCATTTCCTTTGTTTC
PRIMER_RIGHT_SEQUENCE=TCACAATTTAAAACAGGTCATAAAAAC
PRIMER_LEFT=276,22
PRIMER_RIGHT=523,27
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=58.950
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=25.926
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=320.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=248
=
PRIMER_SEQUENCE_ID=F3_X5
TARGET=298,164
SEQUENCE=CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATATCTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAAAGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACGTGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTTAATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAGAAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTACTGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGCCCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCATTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGCTGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTGTTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGAAATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATGTGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
PRIMER_LEFT_EXPLAIN=considered 4694, overlap target 289, GC content failed 4, low tm 2035, high tm 844, ok 1522
PRIMER_RIGHT_EXPLAIN=considered 4397, overlap target 289, GC content failed 28, low tm 1323, high tm 1565, high end compl 4, long poly-x seq 44, ok 1144
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=194.8388
PRIMER_LEFT_PENALTY=69.931898
PRIMER_RIGHT_PENALTY=124.906952
PRIMER_LEFT_SEQUENCE=CCAGATGAATTAAGAACTCTATCTTTT
PRIMER_RIGHT_SEQUENCE=GGCAAATGGCTGGCAGAG
PRIMER_LEFT=268,27
PRIMER_RIGHT=485,18
PRIMER_LEFT_TM=57.068
PRIMER_RIGHT_TM=62.907
PRIMER_LEFT_GC_PERCENT=29.630
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=60.000000
PRIMER_RIGHT_POSITION_PENALTY=120.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=218
=
PRIMER_SEQUENCE_ID=F3_X6
TARGET=298,141
SEQUENCE=CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTTCTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAATTACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGTGAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTTTGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAGAAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGGCTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATGAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGG
PRIMER_LEFT_EXPLAIN=considered 4464, overlap target 289, GC content failed 57, low tm 1831, high tm 878, ok 1409
PRIMER_RIGHT_EXPLAIN=considered 4311, overlap target 289, GC content failed 51, low tm 1766, high tm 895, long poly-x seq 22, ok 1288
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=9.2187
PRIMER_LEFT_PENALTY=5.991812
PRIMER_RIGHT_PENALTY=3.226865
PRIMER_LEFT_SEQUENCE=TTGACTAGATGCCCTTTATTTTTC
PRIMER_RIGHT_SEQUENCE=CAGTAGCTCCAACAGTGCTTC
PRIMER_LEFT=274,24
PRIMER_RIGHT=459,21
PRIMER_LEFT_TM=58.008
PRIMER_RIGHT_TM=57.773
PRIMER_LEFT_GC_PERCENT=33.333
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
=
PRIMER_SEQUENCE_ID=F5_X10
TARGET=59,219
SEQUENCE=TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTTTCAGAAATTCTGAGAATTT
PRIMER_LEFT_EXPLAIN=considered 2597, overlap target 32, GC content failed 195, low tm 1010, high tm 658, high end compl 23, high repeat similarity 8, ok 671
PRIMER_RIGHT_EXPLAIN=considered 2435, overlap target 50, low tm 1068, high tm 632, ok 685
PRIMER_PAIR_EXPLAIN=considered 18, high end compl 16, ok 2
PRIMER_PAIR_PENALTY=288.2375
PRIMER_LEFT_PENALTY=263.479042
PRIMER_RIGHT_PENALTY=24.758462
PRIMER_LEFT_SEQUENCE=TTGCAAATGAAAACAATTTTGAA
PRIMER_RIGHT_SEQUENCE=TGAAAGGTTACTTCAAGGACAAAA
PRIMER_LEFT=23,23
PRIMER_RIGHT=302,24
PRIMER_LEFT_TM=59.521
PRIMER_RIGHT_TM=59.242
PRIMER_LEFT_GC_PERCENT=21.739
PRIMER_RIGHT_GC_PERCENT=33.333
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=260.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
=
PRIMER_SEQUENCE_ID=F7_X1
TARGET=298,68
SEQUENCE=ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCGCTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTGGCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTTCAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACTTTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATCATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCTGCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCAGGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAGGGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGGAGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGGAGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGAGTCT
PRIMER_LEFT_EXPLAIN=considered 3572, overlap target 289, low tm 118, high tm 2723, long poly-x seq 23, ok 419
PRIMER_RIGHT_EXPLAIN=considered 3734, overlap target 289, GC content failed 17, low tm 289, high tm 2735, high end compl 1, ok 403
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=42.8604
PRIMER_LEFT_PENALTY=0.295503
PRIMER_RIGHT_PENALTY=42.564912
PRIMER_LEFT_SEQUENCE=GCAGCACTGCAGAGATTTCA
PRIMER_RIGHT_SEQUENCE=GAGAAAACCTCCCCGGAC
PRIMER_LEFT=278,20
PRIMER_RIGHT=385,18
PRIMER_LEFT_TM=60.296
PRIMER_RIGHT_TM=59.435
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=7.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=40.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=108
=
PRIMER_SEQUENCE_ID=F7_X2
TARGET=298,70
SEQUENCE=TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCACGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGAATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGGCGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGGATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAGGCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTCACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCACCTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAAGGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGGGGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCTTCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGGGCGGTC
PRIMER_LEFT_EXPLAIN=considered 3301, overlap target 289, GC content failed 28, low tm 14, high tm 2710, high end compl 1, long poly-x seq 65, ok 194
PRIMER_RIGHT_EXPLAIN=considered 3601, overlap target 289, GC content failed 16, low tm 390, high tm 2278, long poly-x seq 22, ok 606
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=306.8177
PRIMER_LEFT_PENALTY=304.241567
PRIMER_RIGHT_PENALTY=2.576172
PRIMER_LEFT_SEQUENCE=CATGGCCTTATGACCCCG
PRIMER_RIGHT_SEQUENCE=CAGTGGCAGTCCCTGCTC
PRIMER_LEFT=265,18
PRIMER_RIGHT=385,18
PRIMER_LEFT_TM=62.242
PRIMER_RIGHT_TM=60.576
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=15.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=300.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
=
PRIMER_SEQUENCE_ID=F7_X2'
TARGET=298,165
SEQUENCE=AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGAGCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAACGGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCCGCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGCGGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAGTCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGTTCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCTTCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGCGCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTCTTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGCGGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGCACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATTTGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
PRIMER_LEFT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1097, low tm 35, high tm 3097, high end compl 6, ok 180
PRIMER_RIGHT_EXPLAIN=considered 4704, overlap target 289, GC content failed 1078, low tm 138, high tm 2874, ok 325
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=1385.8022
PRIMER_LEFT_PENALTY=62.138031
PRIMER_RIGHT_PENALTY=1323.664208
PRIMER_LEFT_SEQUENCE=CTTCACGGAACTCGCATTT
PRIMER_RIGHT_SEQUENCE=AGCCAAAGAGACGCAGGC
PRIMER_LEFT=276,19
PRIMER_RIGHT=546,18
PRIMER_LEFT_TM=58.862
PRIMER_RIGHT_TM=61.664
PRIMER_LEFT_GC_PERCENT=47.368
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=60.000000
PRIMER_RIGHT_POSITION_PENALTY=1320.000000
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
=
PRIMER_SEQUENCE_ID=F7_X3
TARGET=298,29
SEQUENCE=CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAACCTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATT
PRIMER_LEFT_EXPLAIN=considered 3344, overlap target 289, GC content failed 2, low tm 424, high tm 1910, ok 719
PRIMER_RIGHT_EXPLAIN=considered 3344, overlap target 289, low tm 284, high tm 2312, high any compl 8, high end compl 13, high repeat similarity 7, ok 431
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=303.6836
PRIMER_LEFT_PENALTY=1.510814
PRIMER_RIGHT_PENALTY=302.172769
PRIMER_LEFT_SEQUENCE=GGCTTGCTCTCTTGTTCCTTC
PRIMER_RIGHT_SEQUENCE=GTTGCAGGCAGGACTGGT
PRIMER_LEFT=277,21
PRIMER_RIGHT=359,18
PRIMER_LEFT_TM=60.511
PRIMER_RIGHT_TM=59.827
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=300.000000
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=83
=
PRIMER_SEQUENCE_ID=F7_X4
TARGET=298,118
SEQUENCE=GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAGCCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAGGGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAACTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
PRIMER_LEFT_EXPLAIN=considered 4234, overlap target 289, GC content failed 2, low tm 537, high tm 2588, ok 818
PRIMER_RIGHT_EXPLAIN=considered 4234, overlap target 289, GC content failed 81, low tm 378, high tm 2891, ok 595
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=6.3238
PRIMER_LEFT_PENALTY=2.096539
PRIMER_RIGHT_PENALTY=4.227266
PRIMER_LEFT_SEQUENCE=GACACCAGCCCACTCCAC
PRIMER_RIGHT_SEQUENCE=GACCCAAAGTGGGGCCTT
PRIMER_LEFT=280,18
PRIMER_RIGHT=433,18
PRIMER_LEFT_TM=60.097
PRIMER_RIGHT_TM=62.227
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
=
PRIMER_SEQUENCE_ID=F7_X5
TARGET=298,145
SEQUENCE=GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCAACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCCACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCACAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGGGGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAGACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTGACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACGGGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGTCCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCTACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCACAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTCAGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCCAACTGCCCACTCGCAGGAATC
PRIMER_LEFT_EXPLAIN=considered 4351, overlap target 289, GC content failed 139, low tm 152, high tm 3259, long poly-x seq 22, ok 490
PRIMER_RIGHT_EXPLAIN=considered 4504, overlap target 289, GC content failed 1, low tm 499, high tm 3030, high repeat similarity 2, ok 683
PRIMER_PAIR_EXPLAIN=considered 20, high any compl 8, high end compl 10, ok 2
PRIMER_PAIR_PENALTY=84.3569
PRIMER_LEFT_PENALTY=62.311131
PRIMER_RIGHT_PENALTY=22.045801
PRIMER_LEFT_SEQUENCE=GGGTGTCCCCTTCCTGTC
PRIMER_RIGHT_SEQUENCE=TGGGACATGAAGCCTGGT
PRIMER_LEFT=277,18
PRIMER_RIGHT=461,18
PRIMER_LEFT_TM=60.311
PRIMER_RIGHT_TM=60.046
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=55.556
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=60.000000
PRIMER_RIGHT_POSITION_PENALTY=20.000000
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=185
=
PRIMER_SEQUENCE_ID=F7_X6
TARGET=298,114
SEQUENCE=GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCCTGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCAGTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCTGGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTTCCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAGTTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAGGCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTCCCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGCTCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAGCACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACGACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTCACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
PRIMER_LEFT_EXPLAIN=considered 3891, overlap target 289, GC content failed 43, low tm 599, high tm 2265, long poly-x seq 44, ok 651
PRIMER_RIGHT_EXPLAIN=considered 3894, overlap target 289, GC content failed 11, low tm 463, high tm 2371, high end compl 1, long poly-x seq 44, ok 715
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=88.8374
PRIMER_LEFT_PENALTY=6.324793
PRIMER_RIGHT_PENALTY=82.512614
PRIMER_LEFT_SEQUENCE=GCATCTTTCTGACTTTTGTTTTACAC
PRIMER_RIGHT_SEQUENCE=ATCCTGAAGCCAGGGGAAG
PRIMER_LEFT=272,26
PRIMER_RIGHT=434,19
PRIMER_LEFT_TM=59.675
PRIMER_RIGHT_TM=61.513
PRIMER_LEFT_GC_PERCENT=34.615
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=80.000000
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=163
=
PRIMER_SEQUENCE_ID=F7_X7
TARGET=298,128
SEQUENCE=CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTGAAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGAGGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGACCCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGCGAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAGGTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGGGTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCGCGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGCTGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAGCAAC
PRIMER_LEFT_EXPLAIN=considered 4032, overlap target 289, GC content failed 6, low tm 577, high tm 2278, long poly-x seq 44, ok 838
PRIMER_RIGHT_EXPLAIN=considered 4181, overlap target 289, GC content failed 10, low tm 236, high tm 3155, high end compl 5, long poly-x seq 22, ok 464
PRIMER_PAIR_EXPLAIN=considered 126, high any compl 28, high end compl 97, ok 1
PRIMER_PAIR_PENALTY=363.4380
PRIMER_LEFT_PENALTY=301.362873
PRIMER_RIGHT_PENALTY=62.075168
PRIMER_LEFT_SEQUENCE=AGCAATGTGACTTCCACACCT
PRIMER_RIGHT_SEQUENCE=CGGACAGGGGAGAGTGGTA
PRIMER_LEFT=262,21
PRIMER_RIGHT=447,19
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=61.075
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=63.158
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=300.000000
PRIMER_RIGHT_POSITION_PENALTY=60.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=186
=
PRIMER_SEQUENCE_ID=F7_X8
TARGET=298,600
SEQUENCE=GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGCATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACACGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCCTTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCATGCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAGGCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCATCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGCACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCTCTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGGACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGGACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCTGTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATGCCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCGCAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAAAGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
PRIMER_LEFT_EXPLAIN=considered 8901, overlap target 289, GC content failed 87, low tm 201, high tm 7523, long poly-x seq 22, ok 779
PRIMER_RIGHT_EXPLAIN=considered 9054, overlap target 289, GC content failed 79, low tm 1105, high tm 6374, high end compl 1, ok 1206
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=447.0465
PRIMER_LEFT_PENALTY=304.949476
PRIMER_RIGHT_PENALTY=142.096979
PRIMER_LEFT_SEQUENCE=GTGGAAAGGGCCTGAGGG
PRIMER_RIGHT_SEQUENCE=TTCTCTCCACAGGCCAGG
PRIMER_LEFT=265,18
PRIMER_RIGHT=922,18
PRIMER_LEFT_TM=62.949
PRIMER_RIGHT_TM=59.903
PRIMER_LEFT_GC_PERCENT=66.667
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=300.000000
PRIMER_RIGHT_POSITION_PENALTY=140.000000
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=658
=
PRIMER_SEQUENCE_ID=HCF2_X2
TARGET=298,909
SEQUENCE=CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAAAGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATCAAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAAGAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGATGCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAGCTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAACATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGCTCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCTCCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGAGGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACATCGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCAGCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTTCAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACCCGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGAACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAATCACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGGGTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGACTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGACCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAAAGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTTCAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTATGTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGACACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGACACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTAGCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCGGTGGC
PRIMER_LEFT_EXPLAIN=considered 12144, overlap target 289, GC content failed 12, low tm 3242, high tm 5342, ok 3259
PRIMER_RIGHT_EXPLAIN=considered 12144, overlap target 289, GC content failed 15, low tm 3651, high tm 4687, high end compl 7, ok 3495
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=43.7623
PRIMER_LEFT_PENALTY=0.056936
PRIMER_RIGHT_PENALTY=43.705322
PRIMER_LEFT_SEQUENCE=TGTGTTCTGTTTTCCCTCCC
PRIMER_RIGHT_SEQUENCE=TGTGAGAACTGTAAAGGTGCCTC
PRIMER_LEFT=278,20
PRIMER_RIGHT=1231,23
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=60.705
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=40.000000
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=954
=
PRIMER_SEQUENCE_ID=HCF2_X3
TARGET=298,278
SEQUENCE=TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAACAACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAACCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGTGGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAGGGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAGGATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATGAGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATGACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAATTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGGTGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTTTTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAAAGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGCCCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCCCTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCTTTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
PRIMER_LEFT_EXPLAIN=considered 5543, overlap target 289, low tm 1266, high tm 2461, long poly-x seq 38, ok 1489
PRIMER_RIGHT_EXPLAIN=considered 5393, overlap target 289, GC content failed 28, low tm 1331, high tm 2335, high any compl 19, high end compl 7, long poly-x seq 67, ok 1317
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=145.7277
PRIMER_LEFT_PENALTY=1.210543
PRIMER_RIGHT_PENALTY=144.517111
PRIMER_LEFT_SEQUENCE=CCTCTTCCTGTGGCCTTTAC
PRIMER_RIGHT_SEQUENCE=GCTCAAAAGAACAAACACACAGT
PRIMER_LEFT=278,20
PRIMER_RIGHT=605,23
PRIMER_LEFT_TM=58.789
PRIMER_RIGHT_TM=58.483
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=140.000000
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=328
=
PRIMER_SEQUENCE_ID=HCF2_X4
TARGET=298,149
SEQUENCE=ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACGAACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGCGGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTTTAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATCATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAGAACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTCCCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTCAGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCCAGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCATGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTAGTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGATCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACATGTGCTGCGGTCTGGGAAATAGCTAC
PRIMER_LEFT_EXPLAIN=considered 4391, overlap target 289, GC content failed 128, low tm 1570, high tm 1096, long poly-x seq 22, ok 1286
PRIMER_RIGHT_EXPLAIN=considered 4238, overlap target 289, GC content failed 20, low tm 420, high tm 2770, long poly-x seq 44, ok 695
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=1.6506
PRIMER_LEFT_PENALTY=0.451656
PRIMER_RIGHT_PENALTY=1.198986
PRIMER_LEFT_SEQUENCE=CCCCCTTTCCTTTTCTGTCT
PRIMER_RIGHT_SEQUENCE=GGTGGACAAGGGAGTGGTT
PRIMER_LEFT=278,20
PRIMER_RIGHT=465,19
PRIMER_LEFT_TM=59.548
PRIMER_RIGHT_TM=59.801
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, MIR2 Repetitive element - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=0.000000
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
=
PRIMER_SEQUENCE_ID=HCF2_X5
TARGET=298,850
SEQUENCE=CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCCCCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAAGTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCTGTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCTGGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAGTTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACCACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTTTTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCCAGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTGTTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACCAATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTTGGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGTGTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCGCGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAAGCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAATTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAAGCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAGCCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGACCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTTACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAAATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATGTGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTCTGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAGTCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTTGACAGA
PRIMER_LEFT_EXPLAIN=considered 11554, overlap target 289, GC content failed 7, low tm 2490, high tm 6121, ok 2647
PRIMER_RIGHT_EXPLAIN=considered 11432, overlap target 289, GC content failed 57, low tm 2518, high tm 5947, high end compl 12, long poly-x seq 20, ok 2589
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=466.5784
PRIMER_LEFT_PENALTY=3.339047
PRIMER_RIGHT_PENALTY=463.239398
PRIMER_LEFT_SEQUENCE=TCTGACAACTTTCCTTTCCAAAC
PRIMER_RIGHT_SEQUENCE=TTGGCTTTTGATATTTGAGCTTC
PRIMER_LEFT=275,23
PRIMER_RIGHT=1193,23
PRIMER_LEFT_TM=59.661
PRIMER_RIGHT_TM=59.761
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=0.000000
PRIMER_RIGHT_POSITION_PENALTY=460.000000
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=919
=
PRIMER_SEQUENCE_ID=HMGCR_X1
TARGET=74,164
SEQUENCE=CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGGATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCCATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACATGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
PRIMER_LEFT_EXPLAIN=considered 2030, GC content failed 67, low tm 611, high tm 842, high any compl 3, high end compl 38, ok 469
PRIMER_RIGHT_EXPLAIN=considered 1490, overlap target 65, low tm 478, high tm 584, ok 363
PRIMER_PAIR_EXPLAIN=considered 11, high end compl 10, ok 1
PRIMER_PAIR_PENALTY=24308.4077
PRIMER_LEFT_PENALTY=302.282278
PRIMER_RIGHT_PENALTY=24006.125439
PRIMER_LEFT_SEQUENCE=TGAGATCTGGAGGATCCAAG
PRIMER_RIGHT_SEQUENCE=TCTTCAAACTTTGGACATTCATAA
PRIMER_LEFT=39,20
PRIMER_RIGHT=237,24
PRIMER_LEFT_TM=57.718
PRIMER_RIGHT_TM=57.875
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=29.167
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=8.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=300.000000
PRIMER_RIGHT_POSITION_PENALTY=24000.000000
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=199
=
PRIMER_SEQUENCE_ID=HMGCR_X2
TARGET=0,112
SEQUENCE=GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTATATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
PRIMER_LEFT_EXPLAIN=considered 770, GC content failed 5, low tm 460, high tm 81, ok 224
PRIMER_RIGHT_EXPLAIN=considered 770, GC content failed 11, low tm 448, high tm 81, high any compl 7, high end compl 1, ok 222
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=49011.4203
PRIMER_LEFT_PENALTY=20002.797253
PRIMER_RIGHT_PENALTY=29008.623090
PRIMER_LEFT_SEQUENCE=GGATGTTTTGAGCAGTGACA
PRIMER_RIGHT_SEQUENCE=AATATATTTTGATCCAAGTTGACGTA
PRIMER_LEFT=0,20
PRIMER_RIGHT=108,26
PRIMER_LEFT_TM=57.203
PRIMER_RIGHT_TM=57.377
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=26.923
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_POSITION_PENALTY=29000.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=109
=
PRIMER_SEQUENCE_ID=HMGCR_X3
TARGET=0,88
SEQUENCE=GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCACTTCTTAGACAAAGAATTGACAGGCTTGA
PRIMER_LEFT_EXPLAIN=considered 530, GC content failed 1, low tm 361, high tm 44, ok 124
PRIMER_RIGHT_EXPLAIN=considered 530, GC content failed 1, low tm 416, high tm 4, ok 109
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=40005.8232
PRIMER_LEFT_PENALTY=19002.290742
PRIMER_RIGHT_PENALTY=21003.532432
PRIMER_LEFT_SEQUENCE=GGTATTGCTGGCCTTTTCA
PRIMER_RIGHT_SEQUENCE=TCAAGCCTGTCAATTCTTTGT
PRIMER_LEFT=0,19
PRIMER_RIGHT=87,21
PRIMER_LEFT_TM=58.709
PRIMER_RIGHT_TM=57.468
PRIMER_LEFT_GC_PERCENT=47.368
PRIMER_RIGHT_GC_PERCENT=38.095
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=19000.000000
PRIMER_RIGHT_POSITION_PENALTY=21000.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=88
=
PRIMER_SEQUENCE_ID=HMGCR_X4
TARGET=0,85
SEQUENCE=ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAAAGTTTGCCCTCAGTTCCAACTCACA
PRIMER_LEFT_EXPLAIN=considered 500, low tm 151, high tm 175, ok 174
PRIMER_RIGHT_EXPLAIN=considered 500, low tm 123, high tm 202, ok 175
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=39005.4820
PRIMER_LEFT_PENALTY=20001.557050
PRIMER_RIGHT_PENALTY=19003.924962
PRIMER_LEFT_SEQUENCE=ATGAAGCTTTGCCCTTTTTC
PRIMER_RIGHT_SEQUENCE=TGTGAGTTGGAACTGAGGG
PRIMER_LEFT=0,20
PRIMER_RIGHT=84,19
PRIMER_LEFT_TM=58.443
PRIMER_RIGHT_TM=57.075
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=52.632
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_POSITION_PENALTY=19000.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=85
=
PRIMER_SEQUENCE_ID=HMGCR_X5
TARGET=0,106
SEQUENCE=GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCTCGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
PRIMER_LEFT_EXPLAIN=considered 710, low tm 212, high tm 246, high end compl 1, ok 251
PRIMER_RIGHT_EXPLAIN=considered 710, low tm 202, high tm 248, high end compl 1, high repeat similarity 1, ok 258
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=47007.3895
PRIMER_LEFT_PENALTY=25005.106680
PRIMER_RIGHT_PENALTY=22002.282868
PRIMER_LEFT_SEQUENCE=GGATGAAGTAAGGGAAAATATTGCT
PRIMER_RIGHT_SEQUENCE=TGACATGGTACCAACTCCAATC
PRIMER_LEFT=0,25
PRIMER_RIGHT=105,22
PRIMER_LEFT_TM=60.107
PRIMER_RIGHT_TM=59.717
PRIMER_LEFT_GC_PERCENT=36.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=25000.000000
PRIMER_RIGHT_POSITION_PENALTY=22000.000000
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
=
PRIMER_SEQUENCE_ID=HMGCR_X6
TARGET=0,107
SEQUENCE=GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTACTTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
PRIMER_LEFT_EXPLAIN=considered 720, low tm 206, high tm 309, ok 205
PRIMER_RIGHT_EXPLAIN=considered 720, low tm 181, high tm 338, ok 201
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=41009.5365
PRIMER_LEFT_PENALTY=18004.862764
PRIMER_RIGHT_PENALTY=23004.673725
PRIMER_LEFT_SEQUENCE=GGGGTACGTCAGCTTGAA
PRIMER_RIGHT_SEQUENCE=TCTAATACCAAGGACACACAAGC
PRIMER_LEFT=0,18
PRIMER_RIGHT=106,23
PRIMER_LEFT_TM=57.137
PRIMER_RIGHT_TM=58.326
PRIMER_LEFT_GC_PERCENT=55.556
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_POSITION_PENALTY=23000.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=107
=
PRIMER_SEQUENCE_ID=HMGCR_X7
TARGET=0,117
SEQUENCE=GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGTTTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
PRIMER_LEFT_EXPLAIN=considered 820, GC content failed 3, low tm 162, high tm 464, ok 191
PRIMER_RIGHT_EXPLAIN=considered 820, GC content failed 3, low tm 237, high tm 349, ok 231
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=59009.6290
PRIMER_LEFT_PENALTY=35003.015981
PRIMER_RIGHT_PENALTY=24006.612999
PRIMER_LEFT_SEQUENCE=GCGAGGGTCGTCCAATTT
PRIMER_RIGHT_SEQUENCE=ATAATCATCTTGACCCTCTGAGTT
PRIMER_LEFT=17,18
PRIMER_RIGHT=116,24
PRIMER_LEFT_TM=61.016
PRIMER_RIGHT_TM=57.387
PRIMER_LEFT_GC_PERCENT=55.556
PRIMER_RIGHT_GC_PERCENT=37.500
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=35000.000000
PRIMER_RIGHT_POSITION_PENALTY=24000.000000
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
=
PRIMER_SEQUENCE_ID=HMGCR_X8
TARGET=0,161
SEQUENCE=GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCAAAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAGAATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
PRIMER_LEFT_EXPLAIN=considered 1260, low tm 540, high tm 321, ok 399
PRIMER_RIGHT_EXPLAIN=considered 1260, low tm 530, high tm 315, high repeat similarity 20, ok 395
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=51007.1128
PRIMER_LEFT_PENALTY=23004.249004
PRIMER_RIGHT_PENALTY=28002.863756
PRIMER_LEFT_SEQUENCE=TCTCTAGGCTTGGTTCTTGTTC
PRIMER_RIGHT_SEQUENCE=GATAAAACTGCCAGAGGGAAAC
PRIMER_LEFT=1,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=57.751
PRIMER_RIGHT_TM=59.136
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_POSITION_PENALTY=28000.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=154
=
PRIMER_SEQUENCE_ID=HMGCR_X9
TARGET=0,248
SEQUENCE=AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTGTCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTATCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTATGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAGAAAGAAAA
PRIMER_LEFT_EXPLAIN=considered 1977, GC content failed 1, low tm 1154, high tm 176, high end compl 1, long poly-x seq 22, ok 623
PRIMER_RIGHT_EXPLAIN=considered 1977, low tm 1125, high tm 203, long poly-x seq 22, ok 627
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=44008.1655
PRIMER_LEFT_PENALTY=23004.538081
PRIMER_RIGHT_PENALTY=21003.627403
PRIMER_LEFT_SEQUENCE=AAATGATCAGCATGGATATTGAA
PRIMER_RIGHT_SEQUENCE=TTTTCTTTCTCGGTTTATCCC
PRIMER_LEFT=0,23
PRIMER_RIGHT=247,21
PRIMER_LEFT_TM=58.462
PRIMER_RIGHT_TM=57.373
PRIMER_LEFT_GC_PERCENT=30.435
PRIMER_RIGHT_GC_PERCENT=38.095
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_POSITION_PENALTY=21000.000000
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
=
PRIMER_SEQUENCE_ID=HMGCR_X10
TARGET=0,90
SEQUENCE=GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTGGTTGGTAACTCCTCCTTACTCGATACTTCA
PRIMER_LEFT_EXPLAIN=considered 550, low tm 222, high tm 109, ok 219
PRIMER_RIGHT_EXPLAIN=considered 550, low tm 227, high tm 116, ok 207
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=49012.4581
PRIMER_LEFT_PENALTY=25006.169796
PRIMER_RIGHT_PENALTY=24006.288343
PRIMER_LEFT_SEQUENCE=TTGAGGTTATAAAACCCTTAGTGG
PRIMER_RIGHT_SEQUENCE=TGAAGTATCGAGTAAGGAGGAGTT
PRIMER_LEFT=1,24
PRIMER_RIGHT=89,24
PRIMER_LEFT_TM=57.830
PRIMER_RIGHT_TM=57.712
PRIMER_LEFT_GC_PERCENT=37.500
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=25000.000000
PRIMER_RIGHT_POSITION_PENALTY=24000.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=89
=
PRIMER_SEQUENCE_ID=HMGCR_X11
TARGET=0,89
SEQUENCE=TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAAGAATGTCTACAGATACTTGGGAATGCAGA
PRIMER_LEFT_EXPLAIN=considered 540, low tm 135, high tm 249, high end compl 1, ok 155
PRIMER_RIGHT_EXPLAIN=considered 540, low tm 181, high tm 215, ok 144
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=44006.1763
PRIMER_LEFT_PENALTY=23002.457721
PRIMER_RIGHT_PENALTY=21003.718624
PRIMER_LEFT_SEQUENCE=CAGTACTGGTGACACAGGAACC
PRIMER_RIGHT_SEQUENCE=TCTGCATTCCCAAGTATCTGT
PRIMER_LEFT=1,22
PRIMER_RIGHT=88,21
PRIMER_LEFT_TM=59.542
PRIMER_RIGHT_TM=57.281
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, ALU Human ALU interspersed repetitive sequence - a  consensus  DO NOT USE THIS LIBRARY FOR PRODUCTION This library contains 4 elements of Jurka's repbase / humrep library. The original is at ftp://ncbi.nlm.nih.gov/repository/repbase.
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_POSITION_PENALTY=21000.000000
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=88
=
PRIMER_SEQUENCE_ID=HMGCR_X12
TARGET=0,195
SEQUENCE=GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATATCCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCGACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGATTATAATTACTCCTT
PRIMER_LEFT_EXPLAIN=considered 1600, low tm 646, high tm 347, ok 607
PRIMER_RIGHT_EXPLAIN=considered 1600, low tm 707, high tm 324, ok 569
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=50013.2868
PRIMER_LEFT_PENALTY=23003.785045
PRIMER_RIGHT_PENALTY=27009.501708
PRIMER_LEFT_SEQUENCE=GAAAGGTGCAAAATTCCTTAGTG
PRIMER_RIGHT_SEQUENCE=AAGGAGTAATTATAATCCCTGTAAGGT
PRIMER_LEFT=0,23
PRIMER_RIGHT=194,27
PRIMER_LEFT_TM=59.215
PRIMER_RIGHT_TM=57.498
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=33.333
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_POSITION_PENALTY=27000.000000
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=195
=
PRIMER_SEQUENCE_ID=HMGCR_X13
TARGET=0,159
SEQUENCE=GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGCAGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTGTCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 1, low tm 314, high tm 591, ok 334
PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 1, low tm 280, high tm 628, ok 331
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=37006.8140
PRIMER_LEFT_PENALTY=18004.525596
PRIMER_RIGHT_PENALTY=19002.288404
PRIMER_LEFT_SEQUENCE=GGTGATGGGAGCTTGTTG
PRIMER_RIGHT_SEQUENCE=CCTATTGCTCTGCAGCCTC
PRIMER_LEFT=0,18
PRIMER_RIGHT=158,19
PRIMER_LEFT_TM=57.474
PRIMER_RIGHT_TM=58.712
PRIMER_LEFT_GC_PERCENT=55.556
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_POSITION_PENALTY=19000.000000
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=159
=
PRIMER_SEQUENCE_ID=HMGCR_X14
TARGET=0,158
SEQUENCE=TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGTGCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGGGTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
PRIMER_LEFT_EXPLAIN=considered 1230, low tm 83, high tm 898, ok 249
PRIMER_RIGHT_EXPLAIN=considered 1230, low tm 122, high tm 805, ok 303
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=38002.8543
PRIMER_LEFT_PENALTY=18002.835611
PRIMER_RIGHT_PENALTY=20000.018700
PRIMER_LEFT_SEQUENCE=TCTTGGTGGAGGTGCCAG
PRIMER_RIGHT_SEQUENCE=TGCTAGTGCTGTCAAATGCC
PRIMER_LEFT=0,18
PRIMER_RIGHT=157,20
PRIMER_LEFT_TM=60.836
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=61.111
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=18000.000000
PRIMER_RIGHT_POSITION_PENALTY=20000.000000
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=158
=
PRIMER_SEQUENCE_ID=HMGCR_X15
TARGET=0,106
SEQUENCE=GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTTTCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
PRIMER_LEFT_EXPLAIN=considered 710, low tm 257, high tm 250, ok 203
PRIMER_RIGHT_EXPLAIN=considered 710, low tm 172, high tm 343, ok 195
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=44006.7460
PRIMER_LEFT_PENALTY=23005.359396
PRIMER_RIGHT_PENALTY=21001.386646
PRIMER_LEFT_SEQUENCE=GATTTGCACGTCTACAGAAACTT
PRIMER_RIGHT_SEQUENCE=TTTGAAATCATGTTCATCCCC
PRIMER_LEFT=0,23
PRIMER_RIGHT=105,21
PRIMER_LEFT_TM=57.641
PRIMER_RIGHT_TM=59.613
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=38.095
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=23000.000000
PRIMER_RIGHT_POSITION_PENALTY=21000.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
=
PRIMER_SEQUENCE_ID=HMGCR_X16
TARGET=0,171
SEQUENCE=GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGCCGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAGAGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
PRIMER_LEFT_EXPLAIN=considered 1360, low tm 684, high tm 181, high end compl 8, high repeat similarity 2, ok 485
PRIMER_RIGHT_EXPLAIN=considered 1360, low tm 616, high tm 248, high end compl 1, ok 495
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=48007.2971
PRIMER_LEFT_PENALTY=28005.907183
PRIMER_RIGHT_PENALTY=20001.389940
PRIMER_LEFT_SEQUENCE=CAGAGAAAGCACTTTCAAAACTT
PRIMER_RIGHT_SEQUENCE=TCTCTGACAACCTTGGCTGG
PRIMER_LEFT=5,23
PRIMER_RIGHT=170,20
PRIMER_LEFT_TM=57.093
PRIMER_RIGHT_TM=61.390
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=28000.000000
PRIMER_RIGHT_POSITION_PENALTY=20000.000000
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
=
PRIMER_SEQUENCE_ID=HMGCR_X17
TARGET=0,141
SEQUENCE=AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGGCTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGCCATCTACATTGCCTGTGGACA
PRIMER_LEFT_EXPLAIN=considered 1060, GC content failed 1, low tm 295, high tm 528, high end compl 3, ok 233
PRIMER_RIGHT_EXPLAIN=considered 1060, GC content failed 1, low tm 217, high tm 601, ok 241
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=50007.1998
PRIMER_LEFT_PENALTY=29005.780871
PRIMER_RIGHT_PENALTY=21001.418918
PRIMER_LEFT_SEQUENCE=AAAGACTACCACAGAGGCTATGA
PRIMER_RIGHT_SEQUENCE=TGTCCACAGGCAATGTAGATG
PRIMER_LEFT=6,23
PRIMER_RIGHT=140,21
PRIMER_LEFT_TM=57.219
PRIMER_RIGHT_TM=59.581
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=18.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=29000.000000
PRIMER_RIGHT_POSITION_PENALTY=21000.000000
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
=
PRIMER_SEQUENCE_ID=HMGCR_X18
TARGET=0,159
SEQUENCE=GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCCCACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGGTGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
PRIMER_LEFT_EXPLAIN=considered 1240, GC content failed 18, low tm 522, high tm 395, high repeat similarity 2, ok 303
PRIMER_RIGHT_EXPLAIN=considered 1240, GC content failed 18, low tm 504, high tm 411, ok 307
PRIMER_PAIR_EXPLAIN=considered 62, high end compl 59, ok 3
PRIMER_PAIR_PENALTY=40003.3443
PRIMER_LEFT_PENALTY=20000.272067
PRIMER_RIGHT_PENALTY=20003.072251
PRIMER_LEFT_SEQUENCE=GGATGCAGCACAGAATGTTG
PRIMER_RIGHT_SEQUENCE=GCAAACAGGCTTGCTGAGG
PRIMER_LEFT=0,20
PRIMER_RIGHT=157,19
PRIMER_LEFT_TM=60.272
PRIMER_RIGHT_TM=62.072
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=20000.000000
PRIMER_RIGHT_POSITION_PENALTY=20000.000000
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=158
=
PRIMER_SEQUENCE_ID=HMGCR_X19
TARGET=0,155
SEQUENCE=GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGCCCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGCAGGACATCTTGTCAAAAGTCACATGATTCACAACA
PRIMER_LEFT_EXPLAIN=considered 1200, low tm 119, high tm 823, ok 258
PRIMER_RIGHT_EXPLAIN=considered 1200, low tm 167, high tm 784, ok 249
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=44006.4573
PRIMER_LEFT_PENALTY=21002.049610
PRIMER_RIGHT_PENALTY=23004.407655
PRIMER_LEFT_SEQUENCE=GATGCTAGGTGTTCAAGGAGC
PRIMER_RIGHT_SEQUENCE=TGTTGTGAATCATGTGACTTTTG
PRIMER_LEFT=0,21
PRIMER_RIGHT=154,23
PRIMER_LEFT_TM=58.950
PRIMER_RIGHT_TM=58.592
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=21000.000000
PRIMER_RIGHT_POSITION_PENALTY=23000.000000
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=155
=
PRIMER_SEQUENCE_ID=HMGCR_X20
TARGET=0,56
SEQUENCE=GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
PRIMER_LEFT_EXPLAIN=considered 444, overlap target 99, low tm 91, high tm 152, ok 102
PRIMER_RIGHT_EXPLAIN=considered 1150, GC content failed 42, low tm 311, high tm 512, high any compl 26, high end compl 3, long poly-x seq 20, ok 236
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_PENALTY=24265.6164
PRIMER_LEFT_PENALTY=24004.237346
PRIMER_RIGHT_PENALTY=261.379041
PRIMER_LEFT_SEQUENCE=GGTCGAAGATCAATTTACAAGACC
PRIMER_RIGHT_SEQUENCE=GCCCATGTTCCAGTTCAGA
PRIMER_LEFT=0,24
PRIMER_RIGHT=87,19
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=59.621
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=52.632
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=9.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=8.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=17.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_POSITION_PENALTY=24000.000000
PRIMER_RIGHT_POSITION_PENALTY=260.000000
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=88
=
