PRIMER_SELF_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_SELF_END=8
PRIMER_FILE_FLAG=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_TASK=pick_pcr_primers
PRIMER_SEQUENCE_ID=pcr_primers
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
TARGET=21,122
PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 3, ok 3
PRIMER_PAIR_PENALTY=3.4323
PRIMER_LEFT_PENALTY=1.117633
PRIMER_RIGHT_PENALTY=2.314641
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT=0,21
PRIMER_RIGHT=160,18
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=61.111
PRIMER_LEFT_SELF_ANY=12.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=4.0864
PRIMER_LEFT_1_PENALTY=1.771733
PRIMER_RIGHT_1_PENALTY=2.314641
PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=160,18
PRIMER_LEFT_1_TM=58.228
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=61.111
PRIMER_LEFT_1_SELF_ANY=10.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=5.0463
PRIMER_LEFT_2_PENALTY=2.731698
PRIMER_RIGHT_2_PENALTY=2.314641
PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=160,18
PRIMER_LEFT_2_TM=57.268
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=61.111
PRIMER_LEFT_2_SELF_ANY=12.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
=
PRIMER_SEQUENCE_ID=pcr_primers_and_io
PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12
PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34
PRIMER_PAIR_EXPLAIN=considered 7, ok 7
PRIMER_PAIR_PENALTY=2.3342
PRIMER_LEFT_PENALTY=2.101896
PRIMER_RIGHT_PENALTY=0.232299
PRIMER_INTERNAL_OLIGO_PENALTY=4.798289
PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT=9,22
PRIMER_RIGHT=247,20
PRIMER_INTERNAL_OLIGO=141,18
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.232
PRIMER_INTERNAL_OLIGO_TM=62.798
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=2.7735
PRIMER_LEFT_1_PENALTY=2.101896
PRIMER_RIGHT_1_PENALTY=0.671597
PRIMER_INTERNAL_OLIGO_1_PENALTY=4.798289
PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=246,20
PRIMER_INTERNAL_OLIGO_1=141,18
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.328
PRIMER_INTERNAL_OLIGO_1_TM=62.798
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=66.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=2.8468
PRIMER_LEFT_2_PENALTY=2.101896
PRIMER_RIGHT_2_PENALTY=0.744925
PRIMER_INTERNAL_OLIGO_2_PENALTY=4.798289
PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=248,20
PRIMER_INTERNAL_OLIGO_2=141,18
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.745
PRIMER_INTERNAL_OLIGO_2_TM=62.798
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=3.0984
PRIMER_LEFT_3_PENALTY=2.866131
PRIMER_RIGHT_3_PENALTY=0.232299
PRIMER_INTERNAL_OLIGO_3_PENALTY=4.798289
PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=247,20
PRIMER_INTERNAL_OLIGO_3=141,18
PRIMER_LEFT_3_TM=58.134
PRIMER_RIGHT_3_TM=60.232
PRIMER_INTERNAL_OLIGO_3_TM=62.798
PRIMER_LEFT_3_GC_PERCENT=38.095
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=3.2356
PRIMER_LEFT_4_PENALTY=3.003259
PRIMER_RIGHT_4_PENALTY=0.232299
PRIMER_INTERNAL_OLIGO_4_PENALTY=4.798289
PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTAGCGTGCGTGGGGTC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=247,20
PRIMER_INTERNAL_OLIGO_4=141,18
PRIMER_LEFT_4_TM=58.997
PRIMER_RIGHT_4_TM=60.232
PRIMER_INTERNAL_OLIGO_4_TM=62.798
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=6.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=left_only
PRIMER_TASK=pick_left_only
TARGET=100,20
SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152
PRIMER_LEFT_PENALTY=0.0408
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT=72,20
PRIMER_LEFT_TM=59.959
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_LEFT_1_PENALTY=0.2717
PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_LEFT_1=64,20
PRIMER_LEFT_1_TM=59.728
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_LEFT_2_PENALTY=0.2717
PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_LEFT_2=60,20
PRIMER_LEFT_2_TM=59.728
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_LEFT_3_PENALTY=0.3125
PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG
PRIMER_LEFT_3=56,20
PRIMER_LEFT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=6.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_LEFT_4_PENALTY=0.4079
PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_LEFT_4=65,20
PRIMER_LEFT_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
=
PRIMER_SEQUENCE_ID=right_only
PRIMER_TASK=PICK_right_only
TARGET=90,10
SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174
PRIMER_RIGHT_PENALTY=0.2917
PRIMER_RIGHT_SEQUENCE=GTGCTTTGCGTTTTGGAAGT
PRIMER_RIGHT=125,20
PRIMER_RIGHT_TM=60.292
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_RIGHT_1_PENALTY=0.7280
PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA
PRIMER_RIGHT_1=75,20
PRIMER_RIGHT_1_TM=59.272
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_RIGHT_2_PENALTY=0.7821
PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT
PRIMER_RIGHT_2=124,20
PRIMER_RIGHT_2_TM=60.782
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_RIGHT_3_PENALTY=1.1251
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
PRIMER_RIGHT_3=76,21
PRIMER_RIGHT_3_TM=60.125
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_RIGHT_4_PENALTY=1.2557
PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
PRIMER_RIGHT_4=79,21
PRIMER_RIGHT_4_TM=60.256
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_RIGHT_4_END_STABILITY=8.2000
=
PRIMER_SEQUENCE_ID=hyb_probe_only
PRIMER_TASK=pick_HYB_probe_ONly
PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=90,20
SEQUENCE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132
PRIMER_INTERNAL_OLIGO_PENALTY=0.2717
PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
PRIMER_INTERNAL_OLIGO=64,20
PRIMER_INTERNAL_OLIGO_TM=59.728
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
PRIMER_INTERNAL_OLIGO_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_1_PENALTY=0.2717
PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG
PRIMER_INTERNAL_OLIGO_1=60,20
PRIMER_INTERNAL_OLIGO_1_TM=59.728
PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_2_PENALTY=0.3125
PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGCACACCCTGCAGGTTTAG
PRIMER_INTERNAL_OLIGO_2=56,20
PRIMER_INTERNAL_OLIGO_2_TM=60.312
PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
PRIMER_INTERNAL_OLIGO_3_PENALTY=0.3751
PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCTGCACCCTGTAAACCC
PRIMER_INTERNAL_OLIGO_3=1,20
PRIMER_INTERNAL_OLIGO_3_TM=60.375
PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
PRIMER_INTERNAL_OLIGO_4_PENALTY=0.4079
PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_INTERNAL_OLIGO_4=65,20
PRIMER_INTERNAL_OLIGO_4_TM=59.592
PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
=
PRIMER_SEQUENCE_ID=left_only
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=0.0408
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT=0,20
PRIMER_LEFT_TM=59.959
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
=
PRIMER_SEQUENCE_ID=right_only
PRIMER_TASK=pick_left_only
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=0.0408
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT=0,20
PRIMER_LEFT_TM=59.959
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
=
PRIMER_SEQUENCE_ID=hyb_probe_only
PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_PENALTY=0.0408
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT=0,20
PRIMER_LEFT_TM=59.959
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
=
